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http://hdl.handle.net/10261/229418
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dc.contributor.author | Moreno-Avitia, Fabián | - |
dc.contributor.author | Utrilla, José | - |
dc.contributor.author | Bolívar, Francisco | - |
dc.contributor.author | Nogales, Juan | - |
dc.contributor.author | Escalante, Adelfo | - |
dc.date.accessioned | 2021-02-12T09:30:04Z | - |
dc.date.available | 2021-02-12T09:30:04Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Applied Microbiology and Biotechnology 104: 10119-10132 (2020) | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10261/229418 | - |
dc.description.abstract | Pseudomonas chlororaphis is a plant-associated bacterium with reported antagonistic activity against different organisms and plant growth–promoting properties. P. chlororaphis possesses exciting biotechnological features shared with another Pseudomonas with a nonpathogenic phenotype. Part of the antagonistic role of P. chlororaphis is due to its production of a wide variety of phenazines. To expand the knowledge of the metabolic traits of this organism, we constructed the first experimentally validated genome-scale model of P. chlororaphis ATCC 9446, containing 1267 genes and 2289 reactions, and analyzed strategies to maximize its potential for the production of phenazine-1-carboxamide (PCN). The resulting model also describes the capability of P. chlororaphis to carry out the denitrification process and its ability to consume sucrose (Scr), trehalose, mannose, and galactose as carbon sources. Additionally, metabolic network analysis suggested fatty acids as the best carbon source for PCN production. Moreover, the optimization of PCN production was performed with glucose and glycerol. The optimal PCN production phenotype requires an increased carbon flux in TCA and glutamine synthesis. Our simulations highlight the intrinsic H2O2 flux associated with PCN production, which may generate cellular stress in an overproducing strain. These results suggest that an improved antioxidative strategy could lead to optimal performance of phenazine-producing strains of P. chlororaphis. | - |
dc.description.sponsorship | This work was supported by Dirección General de Asuntos del Personal Académico- Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (DGAPA-PAPIIT), Universidad Nacional Autónoma de México, grant IN211420. | - |
dc.language | eng | - |
dc.publisher | Springer Nature | - |
dc.rights | closedAccess | - |
dc.subject | Pseudomonas chlororaphis | - |
dc.subject | Metabolic reconstruction | - |
dc.subject | Genome-scale model | - |
dc.subject | Phenazine-1-carboxamide | - |
dc.subject | Metabolic engineering | - |
dc.title | Metabolic reconstruction of Pseudomonas chlororaphis ATCC 9446 to understand its metabolic potential as a phenazine-1-carboxamide-producing strain | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1007/s00253-020-10913-4 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1007/s00253-020-10913-4 | - |
dc.identifier.e-issn | 1432-0614 | - |
dc.date.updated | 2021-02-12T09:30:05Z | - |
dc.contributor.funder | Universidad Nacional Autónoma de México | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100005739 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
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