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Characterization of cholesteryl oleate-loaded cationic solid lipid nanoparticles for the targeted delivery of nucleic acids

AuthorsSuñé-Pou, Marc; Prieto-Sánchez, Silvia; El Yousfi, Younes; Nardi-Ricart, Anna; Nofrerias-Roig, Isaac; Pérez-Lozano, Pilar; García-Montoya, Encarna; Minarro-Carmona, Montserrat; Ticó, J.R.; Hernández-Munain, Cristina; Suñé-Negre, Josep Mª; Suñé, Carlos
Issue Date24-Sep-2018
AbstractNanoparticles have received considerable attention as vectors for gene delivery. However, the clinical translation of nanoparticles is limited due to poor delivery, immunogenicity, toxicity, high processing costs, and production scaling problems. In recent years, cationic solid lipid nanoparticles (SLNs) have gained considerable attention owing to their advantages over viral and other nanoparticle delivery systems. The use of the cell membrane component cholesterol in lipoplex formulations has attracted interest for successful transfection, thus opening new avenues for the synthesis of novel carriers using cholesterol or its derivatives, which could greatly enhance the delivery and activity of nucleic acids. Here, we describe the development and characterization of a stable and scalable formulation of SLNs containing cholesteryl oleate. In addition to studying the physicochemical properties of the SLNs, the effect of cholesteryl oleate on the cytotoxicity, plasmid DNA and RNA transfection efficiency, and gene inhibition capacity of the lipoplexes was also studied. We identified a formula optimized in terms of structure, morphology, and nucleic acid binding efficiency without cytotoxicity, as well as superior transfection efficiency for inducing potent biological activity.
Appears in Collections:(IPBLN) Comunicaciones congresos
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