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Título

Isolation and characterization of a mutant of Saccharomyces cerevisiae defective in phosphoenolpyruvate carboxykinase

AutorPerea, Javier; Gancedo, Carlos
Palabras clavePhosphoenolpyruvate carboxykinase
Gluconeogenesis
saccharomyces cerevisiae
Mutant
Fecha de publicación1982
EditorSpringer
CitaciónArchives of Microbiology 132(2): 141-143 (1982)
ResumenA mutant of Saccharomices cerevisiae lacking phosphoenolpyruvate carboxykinase (E.C. 4.1.1.32) was isolated. The mutant did not grow on gluconeogenic sources except glycerol. The mutation was recessive and apparently affected the structural gene of the enzyme. Intracellular levels of metabolites related to the metabolic situation of the enzyme were not significantly affected after trasfer of the mutant from a medium with glycerol to a medium with ethanol as carbon source. In these conditions only AMP decreased 3 to 5 times. A search for mutants affected in the other gluconeogenic enzyme, fructose 1,6 biphosphatase, remained unsuccessful.
DescripciónPubmed PMID: 6751258
Versión del editorhttp://link.springer.com/article/10.1007%2FBF00508719#
URIhttp://hdl.handle.net/10261/22846
ISSN0302-8933
ReferenciasPE228
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