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Título

In situ study of the glycolytic pathway in Saccharomyces cerevisiae

AutorBañuelos, Marcelino; Gancedo, Carlos
Palabras claveSaccharomyces cerevisiae
Glycolytic pathway
Fermentation rate
Protein concentration
Kinetic parameters
Glycolytic enzymes
Fecha de publicación1978
EditorSpringer
CitaciónArchives of Microbiology 117(2): 197-201 (1978)
Resumen1. The problem of the influence of protein concentration on the kinetic parameters of enzymes has been approached studying the glycolytic enzymes from Saccharomyces cerevisiae in permeabilized cells (in situ). 2. The values of Km and Vmax for the different enzymes were essentially the same in dilute solutions of protein and in concentrated ones (in situ) except in the case of enolase where some differences were observed. 3. Functioning of the whole glycolytic pathway was compared in situ and in vitro measuring the rate of the fermentation of glucose. The rate of fermentation in situ was two fold higher than in vitro and the lag before active fermentation was also much shorter. 4. An unidentified phosphorylated compound, possibly polyphosphate, accumulates during the fermentation of glucose under in situ conditions
DescripciónLos autores pertenecen al antiguo Instituto de Enzimología del CSIC.
Versión del editorhttp://dx.doi.org/10.1007/BF00402308
URIhttp://hdl.handle.net/10261/22844
DOI10.1007/BF00402308
ISSN0302-8933
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