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Epigenetic heterogeneity among modules of individual Lavandula latifolia plants

AuthorsHerrera, Carlos M. CSIC ORCID; Bazaga, Pilar CSIC ORCID; Pérez, Ricardo CSIC ORCID; Alonso, Conchita CSIC ORCID
KeywordsEpigenetic mosaicism
DNA methylation
Genealogical signal,
Lavandula latifolia
Methylation-sensitive amplified fragment length polymorphism (MS-AFLP)
Subindividual variation
Within-plant variation
Issue Date2021
CitationHerrera, Carlos M.; Bazaga, Pilar; Pérez, Ricardo; Alonso, Conchita; 2021; Epigenetic heterogeneity among modules of individual Lavandula latifolia plants [Dataset], 2021
AbstractThis dataset includes: (i) global DNA cytosine methylation estimates (= percentage of all genomic cytosines that are methylated), obtained by reverse phase HPLC with spectrofluorimetric detection; and (ii) the scoring (presence/absence) of individual methylation-sensitive AFLP markers (MS-AFLP), for different modules of three adult, wild-grown, field-sampled plants of the shrub Lavandula latifolia.
DescriptionThe dataset consists of two comma-delimited plain text files for global methylation and MS-AFLP data, respectively. In each file, each row refers to an individual module. For MS-AFLP columns refers to a specific (although anonymous) marker, identified by primer combination and size (base pairs). Three L. latifolia shrubs were sampled at a large population growing near Arroyo Aguaderillos in the Sierra de Cazorla (Jaén province, southeastern Spain; geographical coordinates 37.96157 N, 2.88389 W). Fresh leaf samples were collected from as many individual modules as possible in each plant. DNA cytosine methylation was determined for each sample by reversed phase HPLC with spectrofluorimetric detection. Global cytosine methylation was estimated as 100 x 5mdC/(5mdC + dC), where 5mdC and dC are the integrated areas under the peaks for 5-methyl-2'-deoxycytidine and 2'-deoxycytidine, respectively. The MS-AFLP analyses were performed using standard protocols involving the use of fluorescent dye-labeled selective primers. Each sample was fingerprinted using eight primer combinations, each with two (HpaII) or three (MseI) selective nucleotides. Fragment separation and detection was made using an ABI PRISM 3130xl DNA sequencer, only fragments ≥ 150 base pairs in size were considered and the presence (1) or absence (0) of fragments in each sample was scored manually by visualizing electropherograms with GeneMapper 3.7 software
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