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Título: | Analysis of arbuscular mycorrhizal fungal communities by terminal restriction fragment length polymorphism (TRFLP) |
Autor: | López-García, A. | Palabras clave: | Community assembly Fingerprinting Operational taxonomic units Phylogenetic analyses, R software TRAMPR package |
Fecha de publicación: | 2020 | Editor: | Springer Nature | Citación: | Arbuscular Mycorrhizal Fungi. Methods and Protocols: 117-136 (2020) | Resumen: | Terminal restriction fragment length polymorphism (TRFLP) approaches enable the detection and identification of microbial taxa into samples coming from root or soil material DNA extraction. The low taxonomic diversity of arbuscular mycorrhizal (AM) fungi makes this technique a cheap and adequate method for fingerprinting their communities. Here, I describe the TRFLP database approach, a version of the technique in which the AM fungal taxa present in the sample pool is identified for, later, match their presence in the different samples contained in the experiment. A final AM fungal operational taxonomic unit x sample presence–absence matrix is obtained, which allows the subsequent multivariate statistical analysis of the experimental results. | Descripción: | Ferrol, N.; Lanfranco, L. (Eds.) 2020 Arbuscular Mycorrhizal Fungi. Methods and Protocols. Vol 2146. pp. 257. ISBN 978 1 0716-0602-5 | Versión del editor: | http:///dx.doi.org/10.1007/978-1-0716-0603-2_10 | URI: | http://hdl.handle.net/10261/227390 | DOI: | 10.1007/978-1-0716-0603-2_10 |
Aparece en las colecciones: | (EEZ) Libros y partes de libros |
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