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Title

A nuclear and membrane oestrogen receptor assay to test the oestrogenic activities of pollutants in fish

AuthorsMoreira, Catarina; Zapater, Cinta ; Pinto, Patrícia I. S.; Knigge, Thomas; Gómez, Ana ; Monsinjon, Tiphaine
Issue Date26-May-2019
CitationSociety of Environmental Toxicology and Chemistry Europe - SETAC Europe 29th Annual Meeting (2019)
AbstractOestrogenic compounds are well known endocrine disruptors. In aquatic ecosystems, these compounds may derive from natural (e.g. phytoestrogens) or anthropogenic sources (e.g. pharmaceutical products such as contraceptives or antidepressants) and a continuous exposition to these pollutants may threaten animals’ health. Oestrogens are important regulators of multiple physiological processes and oestrogenic actions are mediated across vertebrates by binding to several oestrogen receptor types. Specific transcription factors, designated nuclear oestrogen receptors (Esrs), have been mainly associated with the classical activation of gene expression by oestrogens or structurally similar compounds. More recently, membrane receptors such as the G protein-coupled oestrogen receptor (Gper) have been associated with rapid, non-genomic responses to estrogens. Both signaling pathways are present in fish, which express additional gene duplicates for both receptor types, although relative oestrogenic activities to the different receptors may differ between species. Using transient transfections in the human embryonic kidney cell line HEK293 and an ERE-luciferase reporter gene assay, the activation of sea bass Esrs was studied in response to of two compounds, the phytoestrogen genistein and the antidepressant and emerging pollutant fluoxetine (Prozac®). In addition, the activation of the two recently identified sea bass Gper duplicates was evaluated using a cAMP response element-luciferase reporter gene assay. Results indicate that genistein and fluoxetine affect each nuclear receptor in a different manner: Fluoxetine rather triggers an anti-oestrogenic response, while genistein behaves as an oestrogen mimic in the transactivation of the three Esrs. Oestradiol was able to induce luciferase activity in cells transfected with both membrane receptors, confirming, for the first time, that both teleost gene duplicates are functional. Furthermore, several xenoestrogens also stimulated luciferase activity and G1 and G15 were confirmed as agonist and antagonist of sea bass Gper, respectively. This study also demonstrates the suitability of the luciferase reporter assay as a tool to assess the oestrogenic potency and mechanisms of action of a wide variety of chemicals. Potential risks of the exposure of fish to various compounds in the environment or in aquaculture can be efficiently identified.
DescriptionComunicación presentada en el Society of Environmental Toxicology and Chemistry Europe - SETAC Europe 29th Annual Meeting, celebrado en Helsinki (Finlandia) del 26 al 30 de mayo de 2019.
URIhttp://hdl.handle.net/10261/227143
ISSN2309-8031
E-ISSN2310-3043
Appears in Collections:(IATS) Comunicaciones congresos
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