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Preparation and metabolic characterization of isolated rat lung cells

AuthorsPérez Díaz, Julio ; Carballo, B.; Sánchez Ayuso, Matilde ; Parrilla, Roberto L.
Issue Date12-Jun-1977
CitationBiochimie 59(4): 411-416 (1977)
AbstractCollagenase digestion of minced lung tissue yielded isolated cells, functionally viable as judged by several metabolic and morphological criteria, representative of all the cell species normally present in the tissue. The efficiency of the isolation procedure was about 25 per cent. Aerobic metabolism was not affected by most of the substrates tested except by succinate which increased oxygen utilization, and glucose, fructose and octanoate which significantly decreased oxygen uptake. Since no significant changes have been observed in the cellular adenine nucleotide content during glucose depression of aerobic metabolism it is concluded that the glycolytic flux had to be sufficient as to account for the decrease in the mitochondrial energy production. The mechanism responsible for these effects as well as their physiological significance are discussed herewith.
Publisher version (URL)https://doi.org/10.1016/S0300-9084(77)80317-9
Identifiersdoi: 10.1016/S0300-9084(77)80317-9
issn: 0300-9084
Appears in Collections:(CIB) Artículos
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