English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/22625
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Purification and characterization of a P-selectin-binding molecule from thesalivary glands of Ornithodoros moubata that induces protective anti-tick immune responses in pigs.

AutorGarcía Varas, Susana; Manzano Román, Raúl ; Fernández Soto, Pedro; Encinas Grandes, Antonio; Oleaga, Ana ; Pérez Sánchez, Ricardo
Palabras claveOrnithodoros moubata
Tick
Salivary antigens
Anti-haemostatics
P-selectin
Heparin
Pig
Vaccine
Fecha de publicación2010
EditorElsevier
CitaciónInt J Parasitol. 2010 Mar 1;40(3):313-26.
ResumenOrnithodoros moubata is an argasid tick that lives in Africa in wild and synanthropic habitats. It feeds on warthogs, domestic swine and humans, and is able to transmit severe diseases such as human relapsing fever and African swine fever. The elimination of O. moubata from the synanthropic surroundings should improve control of the diseases it transmits. Previous attempts to develop a vaccine against O. moubata showed that a salivary gland extract (SGE) induced a protective response that inhibited the feeding of the ticks by up to 60%. Our aim in the present work was to isolate and characterize the salivary antigens responsible for the protective immune response induced with the SGE. The work reported here describes the finding and partial characterization of a tick salivary glycoprotein of 44 kDa (Om44) that binds host P selectin, presumably preventing the adhesion of leukocytes and platelets to vessels walls thus allowing the ticks to complete their feeding. Preliminary analysis indicated that Om44 is not a homologue of the mammalian PSGL-1s and lacks sialyl-LewisX, LewisX 47 and LewisY determinants but carries heparin, which is the P-selectin binding motif. Om44 is not recognised by the pig immune system after natural contact with O. moubata, but it can be neutralised by specific vaccine-induced antibodies, resulting in feeding inhibitions of around 50% in adults and nymphs-4, 25% in nymphs-3, 5% in nymphs-2, and 0% in nymphs-1 when they are fed on Om44-vaccinated pigs. In addition, the fecundity of females was inhibited by up to 43.8%. Om44 opens new perspectives for the development of new anti-tick vaccines.
Versión del editorhttp://www.elsevier.com/wps/find/journaldescription.cws_home/353/description#description
URIhttp://hdl.handle.net/10261/22625
ISSN0020-7519
Aparece en las colecciones: (IRNASA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Copia de IJPara09_251 parA DIGITAL.CSIC.fdf1,59 MBUnknownVisualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.