English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/22551
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 33 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

AutorGarcía-Ruiz, Eva ; Maté, Diana M. ; Ballesteros Olmo, Antonio ; Martínez, Ángel T. ; Alcalde Galeote, Miguel
Fecha de publicación18-mar-2010
EditorBioMed Central
CitaciónMicrobial Cell Factories 9:17 (2010)
Resumen[Background] In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with robust high-throughput protocols. The current work describes how to achieve this goal by engineering ligninolytic oxidoreductases (a high-redox potential laccase -HRPL- and a versatile peroxidase, -VP-) functionally expressed in Saccharomyces cerevisiae.
[Results] Taking advantage of the eukaryotic machinery, complex mutant libraries were constructed by different in vivo recombination approaches and explored for improved stabilities and activities. A reliable high-throughput assay based on the analysis of T50 was employed for discovering thermostable oxidases from mutant libraries in yeast. Both VP and HRPL libraries contained variants with shifts in the T50 values. Stabilizing mutations were found at the surface of the protein establishing new interactions with the surrounding residues.
[Conclusions] The existing tradeoff between activity and stability determined from many point mutations discovered by directed evolution and other protein engineering means can be circumvented combining different tools of in vitro evolution.
Descripción44 pages, 8 figures, 1 table.-- Provisional PDF.
Versión del editorhttp://dx.doi.org/10.1186/1475-2859-9-17
Aparece en las colecciones: (CIB) Artículos
(ICP) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
1475-2859-9-17.pdf2,96 MBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.