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dc.contributor.authorSánchez-Marín, Paulaes_ES
dc.contributor.authorVidal Liñán, Leticiaes_ES
dc.contributor.authorFernández González, Laura Emiliaes_ES
dc.contributor.authorMontes, Rosaes_ES
dc.contributor.authorRodil, Rosarioes_ES
dc.contributor.authorQuintana, José Benitoes_ES
dc.contributor.authorCarrera, Mónicaes_ES
dc.contributor.authorMateos, Jesúses_ES
dc.contributor.authorDiz, Ángel P.es_ES
dc.contributor.authorBeiras, Ricardoes_ES
dc.identifier.citationAquatic Toxicology 230: 105688 (2021)es_ES
dc.description15 pages, 6 figures, 3 tableses_ES
dc.description.abstractOrganophosphate flame retardants (OPFRs) are (re-)emergent environmental pollutants increasingly being used because of the restriction of other flame retardants. The chlorinated OPFR, tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is among those of highest environmental concern, but its potential effects in the marine environment have rarely been investigated. We exposed a widely used sentinel marine mussel species, Mytilus galloprovincialis, to 10 μg L−1 of TDCPP during 28 days and studied: (i) the kinetics of bioaccumulation and elimination of the compound, (ii) the effect on two molecular biomarkers, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities, and (iii) proteomic alterations in the gills, following an isobaric labeling quantitative shotgun proteomic approach, at two exposure times (7 and 28 days). Uptake and elimination of TDCPP by mussels were very fast, and the bioconcentration factor of this compound in mussels was 147 L kgww-1, confirming that this compound is not very bioaccumulative, as predicted by its chemical properties. GST activity was not affected by TDCPP exposure, but AChE activity was inhibited by TDCPP at both 7 and 28 days of exposure. Proteomic analysis revealed subtle effects of TDCPP in mussel gills, since few proteins (less than 2 % of the analysed proteome) were significantly affected by TDCPP, and effect sizes were low. The most relevant effects detected were the up-regulation of epimerase family protein SDR39U1, an enzyme that could be involved in detoxification processes, at both exposure times, and the down-regulation of receptor-type tyrosine-protein phosphatase N2-like (PTPRN2) after 7 days of exposure, which is involved in neurotransmitter secretion and might be related to the neurotoxicity described for this compound. Exposure time rather than TDCPP exposure was the most important driver of protein abundance changes, with 33 % of the proteome being affected by this factor, suggesting that stress caused by laboratory conditions could be an important confounding factor that needs to be controlled in similar ecotoxicology studies. Proteomic data are available via ProteomeXchange with identifier PXD019720es_ES
dc.description.sponsorshipL.E. F.-G. is supported with a predoctoral fellowship from Xunta de Galicia (Consellería de Cultura, Educación e Ordenación Universitaria grant ED481A-2017/298) partially co-founded by operative program FSE Galicia 2014-2020. M.C. is supported by the Ramón y Cajal contract (Ministry of Science and Innovation of Spain). This study was funded by Xunta de Galicia (refs. ED431C 2017/36, ED431C 2017/46, ED431C 2020/05 and IN607B 2018/10) and the Spanish Agencia Estatal de Investigación (refs. CTM2017-84763-C3-R-2 and CTM2016-77945-C3), partially co-funded by the European Regional Development Fund (FEDER/ERDF)es_ES
dc.subjectIsobaric labellinges_ES
dc.subjectTandem mass tag (TMT)es_ES
dc.subjectFlame retardantses_ES
dc.subjectMarine musselses_ES
dc.titleProteomic analysis and biochemical alterations in marine mussel gills after exposure to the organophosphate flame retardant TDCPPes_ES
dc.description.peerreviewedPeer reviewedes_ES
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