English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/22516
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Synthesis of peptide sequences related to thrombospondin: factors affecting aspartimide by-product formation

AutorCebrián, Just; Domingo, Víctor M.; Reig Isart, Francesca
Palabras claveAdhesion
Peptide synthesis
Fecha de publicación19-nov-2003
CitaciónJournal of Peptide Research 62(6): 238-244 (2003)
ResumenAspartimide formation is one of the most common secondary reactions on solid phase peptide synthesis. In the present work, we describe the optimization of the synthesis of two thrombospondin fragments containing an Asp-Gly sequence that show a strong tendency to form cyclic aspartimide derivatives in an unusual high percentage. Several different strategies were applied changing type of resin, Fmoc-deprotection reagents, coupling additives, resin cleavage cocktails and the use of Hmb-Gly derivative to minimize the extension of this byproduct. Best results were obtained with cross-linked ethoxylate acrylate (CLEAR®-cross-linked ethoxylate Acrylate Resin)-type resin and pip/dimethylformamide deprotection. Besides, as in biological assays the aspartimide containing sequence resulted to be more active than the linear one, the optimization of its synthesis was also carried out.
Descripción7 pages, 3 figures, 1 table.-- PMID: 14632926 [PubMed].-- Printed version published Dec 2003
Versión del editorhttp://dx.doi.org/10.1046/j.1399-3011.2003.00093.x
Aparece en las colecciones: (IQAC) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.