English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/22499
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
 |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

The Leishmania infantum PUF proteins are targets of the humoral response during visceral leishmaniasis

AutorFolgueira Fernández, Cristina ; Martínez Bonet, Marta; Requena, José María
Fecha de publicación21-ene-2010
EditorBioMed Central
CitaciónBMC Research Notes 3:13 (2010)
ResumenBackground: RNA-binding proteins of the PUF family share a conserved domain consisting of tandemly repeated 36-40 amino acid motifs (typically eight) known as Puf repeats. Proteins containing tandem repeats are often dominant targets of humoral responses during infectious diseases. Thus, we considered of interest to analyze whether Leishmania PUF proteins result antigenic during visceral leishmaniasis (VL).
Findings: Here, employing whole-genome databases, we report the composition, and structural features, of the PUF family in Leishmania infantum. Additionally, the 10 genes of the L. infantum PUF family were cloned and used to express the Leishmania PUFs in bacteria as recombinant proteins. Finally, the antigenicity of these PUF proteins was evaluated by determining levels of specific antibodies in sera from experimentally infected hamsters. The Leishmania PUFs were all recognized by the sera, even though with different degree of reactivity and/or frequency of recognition. The reactivity of hamster sera against recombinant LiPUF1 and LiPUF2 was particularly prominent, and these proteins were subsequently assayed against sera from human patients. High antibody responses against rLiPUF1 and rLiPUF2 were found in sera from VL patients, but these proteins resulted also recognized by sera from Chagas’ disease patients.
Conclusion: Our results suggest that Leishmania PUFs are targets of the humoral response during L. infantum infection and may represent candidates for serodiagnosis and/or vaccine reagents; however, it should be kept in mind the cross-reactivity of LiPUFs with antibodies induced against other trypanosomatids such as Trypanosoma cruzi.
Descripción8 pages, 3 figures, 1 table, 1 additional file.
Versión del editorhttp://dx.doi.org/10.1186/1756-0500-3-13
URIhttp://hdl.handle.net/10261/22499
DOI10.1186/1756-0500-3-13
ISSN1756-0500
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
1756-0500-3-13.pdf582,44 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.