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Copper modulates nitrous oxide emissions from soybean root nodules

AuthorsTortosa Muñoz, Germán; Pacheco, Pedro J.; Hidalgo-García, Alba; Granados, Arsenio; Delgado Huertas, Antonio ; Mesa, Socorro; Bedmar, Eulogio J.; Delgado Igeño, María Jesús
Issue Date6-Sep-2020
PublisherElsevier BV
CitationEnvironmental and Experimental Botany 180: 104262 (2020)
AbstractAgriculture is an important source of the greenhouse gas nitrous oxide (NO) due to the over- or non-synchronised application of nitrogen to crops. Symbiotic nitrogen fixation (SNF) by legume-rhizobia symbiosis can be an effective strategy for NO mitigation, but several environmental factors such as copper (Cu) availability might affect NO emissions derived from legume crops. The aim of this research was to study how Cu can modulate NO emissions by soybean root nodules. Soybean plants inoculated with Bradyrhizobium diazoefficiens USDA110 were grown in the presence of 4 mM KNO and a battery of Cu concentrations added during growth (0, 5, 10, 20, 40, 60 or 100 μM). NO emissions were measured by gas chromatography in both, nodulated roots and detached nodules, after root flooding. Also, N isotope dilution was assayed for SNF and NO determinations. Results showed that an excess of added Cu during growth significantly affected plant physiology, nodulation and SNF of the soybean-B.diazoefficiens symbiosis, being 20 μM the threshold that soybean plants can tolerate without suffering Cu stress. Meanwhile, Cu addition reduced statistically NO emissions by soybean nodules. This reduction was correlated with Cu accumulation in nodules, which affected the denitrifying enzymes activities. Cu excess produced a simultaneous decrease of nitrate and nitrite reductase activities, but an increase of nitrous oxide reductase activity. Finally, the modulation of bacteroidal nitrate reductase activity is proposed as an effective target for the strategies for mitigation of NO emissions derived from soybean crops, probably more effective than nitrous oxide reductase activity.
Publisher version (URL)http://dx.doi.org/10.1016/j.envexpbot.2020.104262
Identifiersdoi: 10.1016/j.envexpbot.2020.104262
issn: 0098-8472
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