English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/22483
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Adherens junctions connect stress fibers between adjacent endothelial cells

AuthorsMillán, Jaime ; Cain, Rob J.; Reglero-Real, Natalia ; Bigarella, Carolina; Marcos Ramiro, Beatriz ; Fernández Martín, Laura ; Correas, Isabel ; Ridley, Anne J.
Issue Date2-Feb-2010
PublisherBioMed Central
CitationBMC Biology
Abstract[Background] Endothelial cell-cell junctions maintain endothelial integrity and regulate vascular morphogenesis and homeostasis. Cell-cell junctions are usually depicted with a linear morphology along the boundaries between adjacent cells and in contact with cortical F-actin. However, in the endothelium, cell-cell junctions are highly dynamic and morphologically heterogeneous.
[Results] We report that endothelial cell-cell junctions can attach to the ends of stress fibres instead of to cortical F-actin, forming structures that we name discontinuous adherens junctions (AJ). Discontinuous AJ are highly dynamic and are increased in response to tumour necrosis factor (TNF)-α, correlating with the appearance of stress fibres. We show that vascular endothelial (VE)-cadherin/β-catenin/α-catenin complexes in discontinuous AJ are linked to stress fibres. Moreover, discontinuous AJ connect stress fibres from adjacent cells independently of focal adhesions, of which there are very few in confluent endothelial cells, even in TNF- α−stimulated cells. RNAi-mediated knockdown of VE-cadherin, but not zonula occludens-1, reduces the linkage of stress fibres to cell-cell junctions, increases focal adhesions, and dramatically alters the distribution of these actin cables in confluent endothelial cells.
[Conclusions] Our results indicate that stress fibres from neighbouring cells are physically connected through discontinuous AJ, and that stress fibres can be stabilized by AJ-associated multiprotein complexes distinct from focal adhesions.
Description31 pages, 10 figures, 1 additional file.-- Provisional PDF.
Publisher version (URL)http://dx.doi.org/10.1186/1741-7007-8-11
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
1741-7007-8-11.pdf5,72 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.