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dc.contributor.authorGómez Pastor, Rocío-
dc.contributor.authorPérez Torrado, Roberto-
dc.contributor.authorCabiscol, Elisa-
dc.contributor.authorRos, Joaquim-
dc.contributor.authorMatallana, Emilia-
dc.identifier.citationMicrobial Cell Factories 9:9 (2010)en_US
dc.description14 pages, 7 figures, 2 tables.en_US
dc.description.abstract[Background] Wine Saccharomyces cerevisiae strains, adapted to anaerobic must fermentations, suffer oxidative stress when they are grown under aerobic conditions for biomass propagation in the industrial process of active dry yeast production. Oxidative metabolism of sugars favors high biomass yields but also causes increased oxidation damage of cell components. The overexpression of the TRX2 gene, coding for a thioredoxin, enhances oxidative stress resistance in a wine yeast strain model. The thioredoxin and also the glutathione/glutaredoxin system constitute the most important defense against oxidation. Trx2p is also involved in the regulation of Yap1pdriven transcriptional response against some reactive oxygen species.en_US
dc.description.abstract[Results] Laboratory scale simulations of the industrial active dry biomass production process demonstrate that TRX2 overexpression increases the wine yeast final biomass yield and also its fermentative capacity both after the batch and fed-batch phases. Microvinifications carried out with the modified strain show a fast start phenotype derived from its enhanced fermentative capacity and also increased content of beneficial aroma compounds. The modified strain displays an increased transcriptional response of Yap1p regulated genes and other oxidative stress related genes. Activities of antioxidant enzymes like Sod1p, Sod2p and catalase are also enhanced. Consequently, diminished oxidation of lipids and proteins is observed in the modified strain, which can explain the improved performance of the thioredoxin overexpressing strain.en_US
dc.description.abstract[Conclusions] We report several beneficial effects of overexpressing the thioredoxin gene TRX2 in a wine yeast strain. We show that this strain presents an enhanced redox defense. Increased yield of biomass production process in TRX2 overexpressing strain can be of special interest for several industrial applications.en_US
dc.description.sponsorshipWe are grateful with Dr. A.K. Bachhawat that kindly donate us YEp352-GSH1 plasmid. This work was supported by grants AGL 2005-00508 and AGL 2008- 00060 from “Ministerio de Educación y Ciencia” (MEC), Spain. R.P.T was a predoctoral fellow of Generalitat Valenciana and R.G-P. is a predoctoral fellow of the I3P program of “Consejo Superior de Investigaciones Científicas” (Spain).en_US
dc.format.extent1030937 bytes-
dc.publisherBioMed Centralen_US
dc.relation.isversionofPublisher’s version-
dc.titleReduction of oxidative cellular damage by overexpression of the thioredoxin TRX2 gene improves yield and quality of wine yeast dry active biomassen_US
dc.description.peerreviewedPeer revieweden_US
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