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Title

The GATA transcription factor Gaf1 represses tRNAs, inhibits growth, and extends chronological lifespan downstream of fission yeast TORC1

AuthorsRodríguez-López, María; Gonzalez, Suam; Hillson, Olivia; Tunnacliffe, Edward; Codlin, Sandra; Tallada, Víctor A. ; Bähler, Jürg; Rallis, Charalampos
KeywordsS. pombe
Cell growth
Aging
Transcription factor
Issue Date2020
PublisherElsevier
CitationCell Reports 30(10): 3240-3249.e4 (2020)
AbstractTarget of Rapamycin Complex 1 (TORC1) signaling promotes growth and aging. Inhibition of TORC1 leads to reduced protein translation, which promotes longevity. TORC1-dependent post-transcriptional regulation of protein translation has been well studied, while analogous transcriptional regulation is less understood. Here we screen fission yeast mutants for resistance to Torin1, which inhibits TORC1 and cell growth. Cells lacking the GATA factor Gaf1 (gaf1Δ) grow normally even in high doses of Torin1. The gaf1Δ mutation shortens the chronological lifespan of non-dividing cells and diminishes Torin1-mediated longevity. Expression profiling and genome-wide binding experiments show that upon TORC1 inhibition, Gaf1 directly upregulates genes for small-molecule metabolic pathways and indirectly represses genes for protein translation. Surprisingly, Gaf1 binds to and downregulates the tRNA genes, so it also functions as a transcription factor for RNA polymerase III. Thus, Gaf1 controls the transcription of both protein-coding and tRNA genes to inhibit translation and growth downstream of TORC1.
Publisher version (URL)https://doi.org/10.1016/j.celrep.2020.02.058
URIhttp://hdl.handle.net/10261/222637
DOI10.1016/j.celrep.2020.02.058
ISSN2211-1247
Appears in Collections:(CABD) Artículos
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