Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/222028
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Title

Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation

AuthorsMoraleda, J.; Caballero-Huertas, Marta; Valdivieso, A. CSIC ORCID; Joly, Sílvia CSIC ORCID ; Ji, J.; Roher, N.; Ribas, Laia CSIC ORCID
KeywordsGonads
Immune
LPS
Methylation
Reproduction
Sex ratio
Zebrafish
Issue DateJan-2021
PublisherElsevier
CitationDevelopmental & Comparative Immunology 114: 103848 (2021)
AbstractInfections are able to trigger epigenetic modifications; however, epigenetic-mediating infections in the immune system in fish is currently unavailable. Within this purpose, zebrafish were immune-stimulated with three lipopolysaccharides (LPS) during sex differentiation. Methylation patterns of three immune genes were studied by a candidate gene approach together with gene expression analysis, and in adulthood, sex ratios were determined. It was shown that the entrance of LPS was through the gills and accumulated in the pronephros. Significant hypomethylation levels of CASP9 and a significant CpG site for IL1β after Pseudomonas aeruginosa LPS exposure were found. No methylation difference was observed for TNFα. Gene expression and correlation data differed among studied genes. Sex ratios showed a feminization in dose and LPS strain-dependent manner. Here, it is provided epigenetic regulatory mechanisms derived by innate response and the first evidence of possible epigenetic interactions between the immune and reproductive systems
Description11 pages, 6 figures, supplementary data https://doi.org/10.1016/j.dci.2020.103848
Publisher version (URL)https://doi.org/10.1016/j.dci.2020.103848
URIhttp://hdl.handle.net/10261/222028
DOI10.1016/j.dci.2020.103848
ISSN0145-305X
Appears in Collections:(ICM) Artículos

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