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Spectroscopic analysis of the interaction of a peptide sequence of Hepatitis G virus with bilayers

AuthorsAlay, Maite; Prat, Josefina; Haro Villar, Isabel ; Rojo, Nuria; Alsina, M. Asunción ; Busquets, M. Antònia
KeywordsHepatitis G
Merocyanine 540
Issue Date12-Mar-2003
CitationTalanta - the International Journal of Pure and Applied Analyt Chemistry 60: 269-277 (2003)
AbstractMerocyanine 540 (MC540) has been used as external probe to determine the interaction of the peptide sequence 125–139 corresponding to the E2 protein of Hepatitis G virus, with lipid bilayers. The probe was incorporated into large unilamellar vesicles (LUVs) or small unilamellar vesicles (SUVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). When incorporated into bilayers, MC540 shows two absorption maxima corresponding to the monomer (570 nm) and dimer (530 nm) form of the probe. Changes in the probe microenvironment are reflected by a modification in the position and/or intensity of these maxima. Addition of increasing amounts of peptide resulted in a slight decrease of the ratio A570/A530 thus indicating a change in MC540 partition into the membrane, going from a hydrophobic to a more hydrophilic environment. This effect was concomitant with an increase in dimer formation as stated from the values of the apparent dimerization constant (Kapp) obtained. Fluorescence spectra as well as steady state anisotropy measurements were in agreement with the above results indicating that the peptide was able to relocate the probe and displacing MC540 from its initial location into the bilayer. Results with SUVs or LUVs were similar for what curvature does not seem to play any role on peptide activity. These results reflect the ability of peptide to interact with biomimetic membranes in the lipid head group region.
Description9 pages, 5 figures, 1 table.-- PMID: 18969049 [PubMed].-- Printed version published Jun, 13, 2003.-- Presented at the 10th International Symposium on Luminescence Spectrometry - Detection Techniques in Flowing Streams - Quality Assurance and Applied Analyis, Spain, Jun 4, 2002.-- Edited by Ana M. Garcia Campana.
Publisher version (URL)http://dx.doi.org/10.1016/S0039-9140(03)00071-7
Appears in Collections:(IQAC) Artículos
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