Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/221471
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk |
Autor: | Roy, Álvaro; Infantes-Lorenzo, José Antonio; Domínguez, Mercedes; Moreno, Inmaculada CSIC ORCID; Pérez, M.; García, N.; García-Seco, Teresa; Álvarez, Julio CSIC ORCID; Romero, Beatriz; Gortázar, Christian CSIC ORCID ; Juan, Lucía de; Domínguez, Lucas CSIC; Bezos, Javier | Palabras clave: | Goat Milk Tuberculosis Diagnosis |
Fecha de publicación: | 2020 | Editor: | Elsevier | Citación: | Research in Veterinary Science 128: 217-223 (2020) | Resumen: | Caprine tuberculosis (TB) is a zoonosis with sanitary and economic repercussions. Caprine TB control programs are based on a test and cull strategy using the intradermal tuberculin tests and slaughterhouse surveillance. However, this approach is not always feasible and may have a limited sensitivity under specific circumstances. In this study, performance of a new experimental test based on the P22 protein complex (P22 ELISA) was evaluated in two TB-infected herds using milk and serum samples and compared with cell-based diagnostic tests. Samples from a low (n = 62, herd 1) and a high (n = 52, herd 2) TB prevalence herd were selected. Moreover, bulk tank milk samples from both herds were analysed using the P22 ELISA. At the end of the study, a group of animals (n = 21) was euthanized and subjected to post-mortem analysis and bacteriological culture. Significant differences (p < .001) on the qualitative and quantitative (ODs) results were observed between herds using both serum and milk samples in the P22 ELISA. The correlation observed in the quantitative results obtained in serum and milk samples was very strong in animals from flock 2 (rs = 0.91) and moderate in animals from flock 1 (rs = 0.46). Among the slaughtered animals, the P22 ELISA detected a higher proportion of lesion-culture positive animals than cell-based diagnostic tests (61.9 and 66.7% using milk and serum samples, respectively). The P22 ELISA using milk samples demonstrated a similar sensitivity compared with serum samples, suggesting it might be a valuable test for TB control in dairy goats. | Versión del editor: | https://doi.org/10.1016/j.rvsc.2019.12.009 | URI: | http://hdl.handle.net/10261/221471 | DOI: | 10.1016/j.rvsc.2019.12.009 | ISSN: | 0034-5288 |
Aparece en las colecciones: | (IREC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
7
checked on 14-mar-2024
WEB OF SCIENCETM
Citations
6
checked on 29-feb-2024
Page view(s)
127
checked on 26-mar-2024
Download(s)
23
checked on 26-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.