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Título

The gluconeogenic enzyme fructose-1,6-bisphosphatase is dispensable for growth of the yeast yarrowia lipolytica in gluconeogenic substrates

AutorJardón, Raquel; Gancedo, Carlos ; Flores, Carmen-Lisset
Palabras claveCarbon catabolite repression
Saccharomyces-cerevisiae
Glucose repression
Isocitrate lyase
Fructose 1,6-bisphosphatase
Fecha de publicación8-ago-2008
EditorAmerican Society for Microbiology
CitaciónEukaryotic Cell 7(10): 1742-1749 (2008)
ResumenThe genes encoding gluconeogenic enzymes in the nonconventional yeast Yarrowia lipolytica were found to be differentially regulated. The expression of Y. lipolytica FBP1 (YlFBP1) encoding the key enzyme fructose-1,6-bisphosphatase was not repressed by glucose in contrast with the situation in other yeasts; however, this sugar markedly repressed the expression of YlPCK1, encoding phosphoenolpyruvate carboxykinase, and YlICL1, encoding isocitrate lyase. We constructed Y. lipolytica strains with two different disrupted versions of YlFBP1 and found that they grew much slower than the wild type in gluconeogenic carbon sources but that growth was not abolished as happens in most microorganisms. We attribute this growth to the existence of an alternative phosphatase with a high Km (2.3 mM) for fructose-1,6-bisphosphate. The gene YlFBP1 restored fructose-1,6-bisphosphatase activity and growth in gluconeogenic carbon sources to a Saccharomyces cerevisiae fbp1 mutant, but the introduction of the FBP1 gene from S. cerevisiae in the Ylfbp1 mutant did not produce fructose-1,6-bisphosphatase activity or growth complementation. Subcellular fractionation revealed the presence of fructose-1,6-bisphosphatase both in the cytoplasm and in the nucleus.
Versión del editorhttp://dx.doi.org/10.1128/EC.00169-08
URIhttp://hdl.handle.net/10261/22136
DOI10.1128/EC.00169-08
ISSN1535-9778
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