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Title

The gluconeogenic enzyme fructose-1,6-bisphosphatase is dispensable for growth of the yeast yarrowia lipolytica in gluconeogenic substrates

AuthorsJardón, Raquel; Gancedo, Carlos CSIC ORCID; Flores, Carmen-Lisset CSIC ORCID
KeywordsCarbon catabolite repression
Saccharomyces-cerevisiae
Glucose repression
Isocitrate lyase
Fructose 1,6-bisphosphatase
Issue Date8-Aug-2008
PublisherAmerican Society for Microbiology
CitationEukaryotic Cell 7(10): 1742-1749 (2008)
AbstractThe genes encoding gluconeogenic enzymes in the nonconventional yeast Yarrowia lipolytica were found to be differentially regulated. The expression of Y. lipolytica FBP1 (YlFBP1) encoding the key enzyme fructose-1,6-bisphosphatase was not repressed by glucose in contrast with the situation in other yeasts; however, this sugar markedly repressed the expression of YlPCK1, encoding phosphoenolpyruvate carboxykinase, and YlICL1, encoding isocitrate lyase. We constructed Y. lipolytica strains with two different disrupted versions of YlFBP1 and found that they grew much slower than the wild type in gluconeogenic carbon sources but that growth was not abolished as happens in most microorganisms. We attribute this growth to the existence of an alternative phosphatase with a high Km (2.3 mM) for fructose-1,6-bisphosphate. The gene YlFBP1 restored fructose-1,6-bisphosphatase activity and growth in gluconeogenic carbon sources to a Saccharomyces cerevisiae fbp1 mutant, but the introduction of the FBP1 gene from S. cerevisiae in the Ylfbp1 mutant did not produce fructose-1,6-bisphosphatase activity or growth complementation. Subcellular fractionation revealed the presence of fructose-1,6-bisphosphatase both in the cytoplasm and in the nucleus.
Publisher version (URL)http://dx.doi.org/10.1128/EC.00169-08
URIhttp://hdl.handle.net/10261/22136
DOI10.1128/EC.00169-08
ISSN1535-9778
Appears in Collections:(IIBM) Artículos

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