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dc.contributor.authorNoher de Halac, I.-
dc.contributor.authorCismondi, Inés Adriana-
dc.contributor.authorRodríguez García, María I.-
dc.contributor.authorFamá, G.-
dc.date.accessioned2007-11-19T15:16:52Z-
dc.date.available2007-11-19T15:16:52Z-
dc.date.issued2003-04-
dc.identifier.citationBiocell (Mendoza), 2003, vol.27, no.1, pp. 11-18.en_US
dc.identifier.issn0327-9545-
dc.identifier.urihttp://hdl.handle.net/10261/2212-
dc.description.abstractCell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used "in situ" immunocytochemical techniques at electron microscopy, involving monoclonal antibodies JIM5 and JIM7 directed against pectin epitopes in fertile pollen grains of Oenothera hookeri.velans ster/+ster. The same region was also analyzed by classical cytochemistry for polysaccharide detection. Immunogold labelling at the JIM7 epitope showed only in mature pollen labelling mainly located at the intine endo-aperture region. Cytoplasmic structures near the plasma membrane of the vegetative cell showed no labelling gold grains. In the same pollen stage the labelling at the JIM5 epitope was mostly confined to a layer located in the limit between the endexine and the ektexine at the level of the border of the oncus. Some tubuli at the base of the ektexine showed also an accumulation of gold particles. No JIM5 label was demonstrated in the aperture chamber and either in any cytoplasmic structure of the pollen grains. The immunocytochemical technique, when compared with the traditional methods for non- cellulose polysaccharide cytochemistry is fare more sensitive and allows the univocal determination of temporal and spatial location of pectins recognized by the JIM7 and JIM5 MAbs.en_US
dc.description.sponsorshipWe are grateful for the grant of the Research Council of Argentina (CONICET). The study was also supported by project no. PGC BMC 2000-1484 from the Spanish Ministerio de Ciencia y Tecnología.en_US
dc.language.isoengen_US
dc.publisherCentro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)en_US
dc.rightsopenAccessen_US
dc.subjectPectinsen_US
dc.subjectMonoclonal antibodiesen_US
dc.subjectJIM5en_US
dc.subjectJIM7en_US
dc.subjectImmunogolden_US
dc.subjectPollenen_US
dc.subjectSporodermen_US
dc.subjectOenotheraen_US
dc.subjectCytochemistryen_US
dc.titleDistribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+steren_US
dc.typeArtículoen_US
dc.description.peerreviewedPeer revieweden_US
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