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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/22128
Título

Pyruvate carboxylase is an essential protein in the assembly of yeast peroxisomal oligomeric alcohol oxidase

AutorOzimek, Paulina; Gancedo, Carlos ; Veenhuis, Marten
Palabras claveFlavin adenine-dinucleotide
Hansenula-polymorpha
Pichia-pastoris
Saccharomyces-cerevisiae
Methylotrophic yeasts
Methanol metabolism
Matrix protein
Gene encodes
Biogenesis
Fecha de publicación18-nov-2002
EditorAmerican Society for Cell Biology
CitaciónMolecular Biology of the Cell 14(2): 786-797 (2003)
ResumenHansenula polymorpha ass3 mutants are characterized by the accumulation of inactive alcohol oxidase (AO) monomers in the cytosol, whereas other peroxisomal matrix proteins are normally activated and sorted to peroxisomes. These mutants also have a glutamate or aspartate requirement on minimal media. Cloning of the corresponding gene resulted in the isolation of the H. polymorpha PYC gene that encodes pyruvate carboxylase (HpPyc1p). HpPyc1p is a cytosolic, anapleurotic enzyme that replenishes the tricarboxylic acid cycle with oxaloacetate. The absence of this enzyme can be compensated by addition of aspartate or glutamate to the growth media. We show that HpPyc1p protein but not the enzyme activity is essential for import and assembly of AO. Similar results were obtained in the related yeast Pichia pastoris. In vitro studies revealed that HpPyc1p has affinity for FAD and is capable to physically interact with AO protein. These data suggest that in methylotrophic yeast pyruvate carboxylase plays a dual role in that, besides its well-characterized metabolic function as anapleurotic enzyme, the protein fulfils a specific role in the AO sorting and assembly process, possibly by mediating FAD-binding to AO monomers
Descripción12 páginas, 10 figuras.-- et al.
Versión del editorhttp://dx.doi.org/10.1091/mbc.E02-07-0417
URIhttp://hdl.handle.net/10261/22128
DOI10.1091/mbc.E02-07-0417
ISSN1059-1524
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