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http://hdl.handle.net/10261/221145
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dc.contributor.author | Aznar-Moreno, José A. | - |
dc.contributor.author | Venegas-Calerón, Mónica | - |
dc.contributor.author | Du, Zhi-Yan | - |
dc.contributor.author | Garcés Mancheño, Rafael | - |
dc.contributor.author | Tanner, Julian A. | - |
dc.contributor.author | Chye, Mee-Len | - |
dc.contributor.author | Martínez-Force, Enrique | - |
dc.contributor.author | Salas, Joaquín J. | - |
dc.date.accessioned | 2020-10-14T10:00:12Z | - |
dc.date.available | 2020-10-14T10:00:12Z | - |
dc.date.issued | 2020 | - |
dc.identifier | doi: 10.1016/j.plantsci.2020.110630 | - |
dc.identifier | issn: 0168-9452 | - |
dc.identifier.citation | Plant Science 300: 110630 (2020) | - |
dc.identifier.uri | http://hdl.handle.net/10261/221145 | - |
dc.description | 5 Figuras.-- 2 Tablas | - |
dc.description.abstract | Acyl-CoA-binding proteins (ACBP) bind to long-chain acyl-CoA esters and phospholipids, enhancing the activity of different acyltransferases in animals and plants. Nevertheless, the role of these proteins in the synthesis of triacylglycerols (TAGs) remains unclear. Here, we cloned a cDNA encoding HaACBP1, a Class II ACBP from sunflower (Helianthus annuus), one of the world's most important oilseed crop plants. Transcriptome analysis of this gene revealed strong expression in developing seeds from 16 to 30 days after flowering. The recombinant protein (rHaACBP1) was expressed in Escherichia coli and purified to be studied by in vitro isothermal titration calorimetry and for phospholipid binding. Its high affinity for saturated palmitoyl-CoA (16:0-CoA; K 0.11 μM) and stearoyl-CoA (18:0-CoA; K 0.13 μM) esters suggests that rHaACBP1 could act in acyl-CoA transfer pathways that involve saturated acyl derivatives. Furthermore, rHaACBP1 also binds to both oleoyl-CoA (18:1-CoA; K 6.4 μM) and linoleoyl-CoA (18:2-CoA; K 21.4 μM) esters, the main acyl-CoA substrates used to synthesise the TAGs that accumulate in sunflower seeds. Interestingly, rHaACBP1 also appears to bind to different species of phosphatidylcholines (dioleoyl-PC and dilinoleoyl-PC), glycerolipids that are also involved in TAG synthesis, and while it interacts with dioleoyl-PA, this is less prominent than its binding to the PC derivative. Expression of rHaACBP in yeast alters its fatty acid composition, as well as the composition and size of the host acyl-CoA pool. These results suggest that HaACBP1 may potentially fulfil a role in the transport and trafficking of acyl-CoAs during sunflower seed development. | - |
dc.description.sponsorship | We thank A. González-Callejas and M. Parra-Camacho for their skilful technical assistance. This work was funded by the MINECO and FEDER Project AGL2017-83449-R, the CSIC/RGC joint research award (Project 2011HK0008; S-HK006/12), and the Wilson and Amelia Wong Endowment Fund. | - |
dc.language | eng | - |
dc.publisher | Elsevier | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83449-R | - |
dc.rights | openAccess | en_EN |
dc.subject | Acyl-CoA | - |
dc.subject | Acyl-CoA-binding protein | - |
dc.subject | Oilseeds | - |
dc.subject | Phosphatidylcholine | - |
dc.subject | Phospholipids | - |
dc.subject | Sunflower | - |
dc.subject | Triacylglycerol | - |
dc.title | Characterization and function of a sunflower (Helianthus annuus L.) Class II acyl-CoA-binding protein | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.plantsci.2020.110630 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.plantsci.2020.110630 | - |
dc.date.updated | 2020-10-14T10:00:12Z | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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PostP_2020_PlantSci_V300_110630.pdf | 2,04 MB | Adobe PDF | Visualizar/Abrir |
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