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Physiological roles of very long‐chain (>C24) fatty acids during early development of fish: Molecular and functional characterization of elovl4 genes in Sparus aurata and Solea senegalensi

AuthorsTorres Rodríguez, M.; Morais, Sofia; Hontoria, Francisco ; Monroig, Óscar ; Varó, Inmaculada ; Agulleiro, María José; Navarro, Juan Carlos
KeywordsSparus aurata
Solea senegalensis
Very long-chain polyunsaturated fatty acids
Nutritional regulation
Issue Date2020
CitationAbstracts International Symposium on Marine Science. Volume VII: 292-293 (2020)
AbstractVery long‐chain fatty acids (VLC‐FA) play critical roles in neural tissues during early development of vertebrates. However, studies on VLC‐FA in fish are scarce. Biosynthesis of VLC‐FA is mediated by elongation of very long‐chain fatty acid 4 (Elovl4) proteins and, consequently, the complement and activity of these enzymes determines the capacity that a given species has for satisfying its physiological demands, in particular for the correct development of neurophysiological functions. The present study aimed to characterize and localize the expression of elovl4 genes from Sparus aurata and Solea senegalensis, as well as to determine the function of their encoded proteins. Results confirmed that both fish possess two distinct elovl4 genes named elovl4a and elovl4b. Functional assays demonstrated that both Elovl4 isoforms had the capability to elongate long‐chain (C20‐24), both saturated (SFA) and polyunsaturated (PUFA), fatty acid precursors to VLC‐FA. In spite of their overlapping activity, Elovl4a was more active in VLC‐SFA elongation, while Elovl4b had a preponderant elongation activity towards n‐3 PUFA substrates, particularly in S. aurata, being additionally the only isoform capable of elongating docosahexaenoic acid (DHA). A preferential expression of elovl4 genes was measured in neural tissues, being elovl4a and elovl4b mRNAs mostly found in brain and eyes, respectively. Importantly, these findings contribute to a better understanding of the VLC‐FA biosynthetic pathway and their physiological role in marine teleosts, specially during early development, when these compounds accumulate in rapidly forming neural tissues
DescriptionTrabajo presentado en el VII International Symposium on Marine Sciences (ISMS), celebrado del 1 al 3 de julio de 2020, en Barcelona (España)
Publisher version (URL)https://isms.cat/wp-content/uploads/2020/09/libro-ISMS-2020.pdf
Appears in Collections:(IATS) Libros y partes de libros
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