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Enzyme-assisted supercritical fluid extraction of antioxidant isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill

AuthorsAntunes-Ricardo, Marilena; Mendiola, J. A. ; García-Cayuela, Tomás ; Ibáñez, Elena ; Gutiérrez-Uribe, Janet A.; Cano, M. Pilar ; Guajardo-Flores, Daniel
KeywordsEnzymatic in situ supercritical fluid extraction
Opuntia ficus-indica
Isorhamnetin conjugates
Issue Date2020
CitationJournal of Supercritical Fluids 158: 104713 (2020)
AbstractA one-pot enzymatic assisted supercritical carbon dioxide (SC-CO2) extraction was used to obtain isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill (nopal). Box-Benhken design, discriminate analysis and principal component analysis were used to studt the influence of pressure, temperature, aqueous ethanol solution and time on the total extraction yield and the isorhamnetin conjugates recovery using the enzymes Rapidase Maxi Fruit (Rapidase) and Viscozyme. Isorhamnetin tri- and diglycoside were found in the extracts along with other phenolic compounds such as 3-O-methyl quercetin, 3-O-methyl kaempferol and luteolin. The isorhamnetin and phenolic profile patterns were dependent on the conditions used. Cellular antioxidant activity was positively correlated to isorhamnetin-3-O-glucosyl-rhamnoside and luteolin content. The best results were obtained by using Rapidase, a currently used enzyme for the winemaking industry. The use of enzymes under SC-CO2 conditions affected the release of isorhamnetin conjugate profiles which induced remarkable differences in the cellular antioxidant activity.
Publisher version (URL)https://doi.org/10.1016/j.supflu.2019.104713
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