English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/22001
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Melanin Production by Rhizobium meliloti GR4 Is Linked to Nonsymbiotic Plasmid pRmeGR4b: Cloning, Sequencing, and Expression of the Tyrosinase Gene mepA

AutorMercado-Blanco, Jesús ; García, Fernando; Fernández-López, Manuel; Olivares Pascual, José
Fecha de publicaciónsep-1993
EditorAmerican Society for Microbiology
CitaciónJournal of Bacteriology 175 (17): 5403-5410 (1993)
ResumenMelanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b (140 MDa). Transfer of this plasmid to GR4-cured derivatives or to Agrobacterium tumefaciens enables these bacteria to produce melanin. Sequence analysis of a 3.5-kb PstI fragment of plasmid pRmeGR4b has revealed the presence of a open reading frame 1,481-bp that codes for a protein whose sequence shows strong homology to two conserved regions involved in copper binding in tyrosinases and hemocyanins. In vitro-coupled transcriptiontranslation experiments showed that this open reading frame codes for a 55-kDa polypeptide. Melanin production in GR4 is not under the control of the RpoN-NifA regulatory system, unlike that in R. leguminosarum bv. phaseoli 8002. The GR4 tyrosinase gene could be expressed in Escherichia coli under the control of the lacZ promoter. For avoiding confusion with mel genes (for melibiose), a change of the name of the previously reported mel genes of R. kguminosarum bv. phaseoli and other organisms to mep genes (for melanin production) is proposed.
Descripción8 pages; 1 table; 5 figures
Versión del editorhttp://jb.asm.org/cgi/reprint/175/17/5403
URIhttp://hdl.handle.net/10261/22001
ISSN0021-9193 (print)
1098-5530 (online)
Aparece en las colecciones: (IAS) Artículos
(EEZ) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.