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Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples

AuthorsVelden, Vincent H. J. van der; Flores-Montero, Juan; Pérez-Andrés, Martin; Martín-Ayuso, Marta; Crespo, Oliver; Blanco, Elena; Kalina, Tomas; Philippé, J.; Bonroy, C.; Bie, Maaike de; Marvelde, Jeroen G. te; Teodosio, Cristina; Corral-Mateos, A.; Kanderová, Veronika; Burg, Mirjam van der; Van Hoof, Dennis; Dongen, J. J. M. van
KeywordsDried antibody tubes
Flow cytometry
Hematological malignancies
Immune deficiencies
Issue Date2019
CitationJournal of Immunological Methods 475: 112287 (2019)
AbstractWithin EuroFlow, we recently developed screening tubes for hematological malignancies and immune deficiencies. Pipetting of antibodies for such 8-color 12-marker tubes however is time-consuming and prone to operational mistakes. We therefore evaluated dried formats of the lymphocytosis screening tube (LST) and of the primary immune deficiency orientation tube (PIDOT). Both tubes were evaluated on normal and/or on patient samples, comparing the mean fluorescence intensity of specific lymphocyte populations. Our data show that the dried tubes and liquid counterparts give highly comparable staining results, particularly when analyzed in multidimensional plots. In addition, the use of dried tubes may result in a reduced staining variability between different samples and thereby contributes to the generation of more robust data. Therefore, by using ready-to-use reagents in a dried single test tube format, the laboratory efficiency and quality will be improved.
Publisher version (URL)https://doi.org/10.1016/j.jim.2017.03.011
Appears in Collections:(IBMCC) Artículos
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