English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/219671
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Frequent issues and lessons learned from EuroFlow QA

AuthorsKalina, Tomas; Brdickova, Nadezda; Glier, Hana; Fernandez, Paula; Bitter, Marieke; Flores-Montero, Juan; Dongen, J. J. M. van; Orfao, Alberto
KeywordsFlow cytometry
Quality assessment scheme
Standardization
EuroFlow
Issue Date2019
PublisherElsevier
CitationJournal of Immunological 475: 112520 (2019)
AbstractEuroFlow Quality Assessment was designed to provide a feedback on the quality of the standardization effort in executing the EuroFlow protocols for sample preparation and instrument setup. It was first beta-tested by the members of the EuroFlow consortium internally (2010−2013) and opened to the external participants from 2015 onwards. The goal of participation in the EuroFlow QA is to evaluate whether the technical quality of the data generated by the laboratory is comparable to the data of the EuroFlow members and thus if a non-EuroFlow member participant can use the EuroFlow reference sample database for his own patient evaluation. Also it assesses whether data are sufficiently standardized for automated population gating and alarm notification. By spring 2018, a total 87 laboratories from 32 countries on five continents have registered for the EuroFlow QA program. We evaluated 163 results of 2015–2016 QA rounds, where we noted clear improvement in the score of first-time participants (median score of 91% correct) when they participated second time or later (median score of 94% correct, p = 0,017), which was comparable to EuroFlow member scores (median score of 97% correct). Among frequent mistakes, we found non-adherence to the EuroFlow protocols (improper reagent used), improper gating and some compensation issues. In summary, we show that EuroFlow QA has a positive impact on improvement of standardized data quality of non-member laboratories adhering to the EuroFlow standard operating procedures and reagent panels.
Publisher version (URL)https://doi.org/10.1016/j.jim.2018.09.008
URIhttp://hdl.handle.net/10261/219671
DOIhttp://dx.doi.org/10.1016/j.jim.2018.09.008
ISSN0022-1759
Appears in Collections:(IBMCC) Artículos
Files in This Item:
File Description SizeFormat 
freQA.pdf6,45 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.