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http://hdl.handle.net/10261/219385
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dc.contributor.author | Zaghetto de Almeida, Paula | es_ES |
dc.contributor.author | Oliveira, Tássio Brito de | es_ES |
dc.contributor.author | Coutinho de Lucas, Rosymar | es_ES |
dc.contributor.author | Santos Salgado, Jose Carlos | es_ES |
dc.contributor.author | Martínez Pérez, Malena | es_ES |
dc.contributor.author | Galán, Beatriz | es_ES |
dc.contributor.author | García, José Luis | es_ES |
dc.contributor.author | Teixeira de Moraes Polizeli, Maria de Lourdes | es_ES |
dc.date.accessioned | 2020-09-09T11:59:20Z | - |
dc.date.available | 2020-09-09T11:59:20Z | - |
dc.date.issued | 2020-12 | - |
dc.identifier.citation | Process Biochemistry 99: 1-8 (2020) | es_ES |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | http://hdl.handle.net/10261/219385 | - |
dc.description.abstract | The enzymatic lignocellulosic biomass conversion into value-added products requires the use of enzyme-rich cocktails, including β-glucosidases that hydrolyze cellobiose and cellooligosaccharides to glucose. During hydrolysis occurs accumulation of monomers causing inhibition of some enzymes; thus, glucose/xylose tolerant β-glucosidases could overcome this drawback. The search of new tolerant enzymes showing additional properties,such as high activity, wide-pH range, and thermal stability is very relevant to improve the bioprocess. We describe a novel β-glucosidase GH1 from the thermophilic Anoxybacillus thermarum (BgAt), which stood out by the robustness combination of great glucose/xylose tolerance, thermal stability, and high Vmax. The recombinant his-tagged-BgAt was overexpressed in Escherichia coli, was purified in one step, showed a high glucose/xylose tolerance, and activity stimulation (presence of 0.4M glucose/1.0M xylose). The optimal activity was at 65 °C - pH 7.0. BgAt presented an extraordinary temperature stability (48 h – 50 °C), and pH stability (5.5–8.0). The novel enzyme showed outstanding Vmax values compared to other β-glucosidases. Using p-nitrophenyl-β-D-glucopyranoside as substrate the values were Vmax (7614 U/mg), and KM (0.360 mM). These values suffer a displacement in Vmax to 14,026 U/mg (glucose), 14,886 U/mg (xylose), and KM 0.877mM (glucose), and 1.410mM (xylose). | es_ES |
dc.description.sponsorship | This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, process no 2018/07522-6) and a scholarship to Tassio B. Oliveira (grant 2017/09000-4). MLTMP is a Research Fellow of Conselho de Desenvolvimento Científico e Tecnológico (CNPq, process 301963/2017-7). The project also received grants from National Institute of Science and Technology of Bioethanol,INCT, CNPq 465319/2014-9/FAPESP nº 2014/50884-5). PZA was a fellow from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) funding code 001 and Programa de Doutorado Sanduiche no Exterior, PDSE no 88881.135684/2016-01. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | β-glucosidase | es_ES |
dc.subject | Anoxybacillus thermarum | es_ES |
dc.subject | Glucose tolerance | es_ES |
dc.subject | Xylose tolerance | es_ES |
dc.title | Heterologous production and biochemical characterization of a new highly glucose tolerant GH1 β-glucosidase from Anoxybacillus thermarum | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.procbio.2020.08.013 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.procbio.2020.08.013 | es_ES |
dc.contributor.funder | Fundação de Amparo à Pesquisa do Estado de São Paulo | es_ES |
dc.contributor.funder | Instituto Nacional de Ciência e Tecnologia do Bioetanol (Brasil) | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100001807 | es_ES |
dc.contributor.orcid | Oliveira, Tássio Brito de [0000-0002-4666-7930] | es_ES |
dc.contributor.orcid | Coutinho de Lucas, Rosymar [0000-0001-8738-7372] | es_ES |
dc.contributor.orcid | Santos Salgado, Jose Carlos [0000-0002-3422-9474] | es_ES |
dc.contributor.orcid | Galán, Beatriz [0000-0002-2596-6034] | es_ES |
dc.contributor.orcid | García, José Luis [0000-0002-9238-2485] | es_ES |
dc.contributor.orcid | Teixeira de Moraes Polizeli, Maria de Lourdes [0000-0002-5026-6363] | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
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