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dc.contributor.authorHou, Weinaes_ES
dc.contributor.authorSingh, Rupesh Kumares_ES
dc.contributor.authorZhao, Panes_ES
dc.contributor.authorMartins, Vivianaes_ES
dc.contributor.authorAguilar, Emmanueles_ES
dc.contributor.authorCanto, Tomáses_ES
dc.contributor.authorTenllado, Franciscoes_ES
dc.contributor.authorFranklin, Gregoryes_ES
dc.contributor.authorDias, Alberto C. P.es_ES
dc.date.accessioned2020-09-09T09:35:40Z-
dc.date.available2020-09-09T09:35:40Z-
dc.date.issued2020-09-02-
dc.identifier.citationJournal of Plant Physiology 153268 (2020)es_ES
dc.identifier.issn0176-1617-
dc.identifier.urihttp://hdl.handle.net/10261/219347-
dc.description43 p.-5 fig.-3 fig. supl.-4 tab. supl.es_ES
dc.description.abstractHypericum perforatum L is a remarkable source of high-value secondary metabolites with increasing applications in pharmaceutical industry. However, improvement in the production of secondary metabolites through genetic engineering is a demanding task, as H. perforatum is not amenable to Agrobacterium tumefaciens-mediated transformation. In this study, we identified a Polygalacturonase-inhibiting protein (PGIP) gene from a subtractive cDNA library of A. tumefaciens-treated H. perforatum suspension cells. The role of HpPGIP in defense against A. tumefaciens was analyzed in transgenic Nicotiana tabacum overexpressing HpPGIP alone or fused at the N-terminus to Phenolic oxidative coupling protein (Hyp-1), a gene that positively modulates resistance to A. tumefaciens. Furthermore, virus-induced gene silencing was employed to knock down the expression of the PGIP homologous in N. benthamiana. Results showed that Agrobacterium-mediated expression efficiency greatly decreased in both HpPGIP and Hyp-1-PGIP transgenic plants, as assessed by GUS staining assays. However, silencing of PGIP in N. benthamiana increased the resistance to A. tumefaciens rather than susceptibility, which correlated with induction of pathogenesis-related proteins (PRs). The expression of core genes involved in several defense pathways was also analyzed in transgenic tobacco plants. Overexpression of HpPGIP led to up-regulation of key genes involved in hormone signaling, microRNA-based gene silencing, homeostasis of reactive oxygen species, and the phenylpropanoid pathway. Overexpression of Hyp-1-PGIP seemed to enhance the effect of PGIP on the expression of most genes analyzed. Moreover, HpPGIP was detected in the cytoplasm, nucleus and the plasma membrane or cell wall by confocal microscopy. Overall, our findings suggest HpPGIP modulates recalcitrance to A. tumefaciens-mediated transformation in H. perforatum.es_ES
dc.description.sponsorshipThe author acknowledges the financial support provided by the Fundação para a Ciência e a Tecnologia(FCT) project (PTDC/AGR-GPL/119211/2010), to G.F., grant UID/BIA/04050/2013 (POCI-01- 0145FEDER-007569), PhD grant (SFRH/BD/52561/2014), under the Doctoral Programme “Agricultural Production Chains – from fork to farm” (PD/00122/2012) to A.C.P., and by Ministry of Economy and Competitiveness of Spain (grant BIO2016-75619-R [AEI/FEDER, UE]) to T.C. and F.T., and by the National Natural Science Foundation of China (31601622) to P.Z.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-75619-Res_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectH. perforatum L. secondary metaboliteses_ES
dc.subjectPlant defense responseses_ES
dc.subjectPathogenesis-related proteinses_ES
dc.subjectPhenolic oxidative coupling proteines_ES
dc.subjectVirus-induced gene silencinges_ES
dc.titleOverexpression of polygalacturonase-inhibiting protein (PGIP) gene from Hypericum perforatum alters expression of multiple defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens in tobaccoes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.jplph.2020.153268-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.jplph.2020.153268es_ES
dc.embargo.terms2022-09-02es_ES
dc.contributor.funderFundação para a Ciência e a Tecnologia (Portugal)es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderNational Natural Science Foundation of Chinaes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001809es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001871es_ES
dc.contributor.orcidHou, Weina [0000-0002-8599-1469]es_ES
dc.contributor.orcidSingh, Rupesh Kumar [0000-0002-2536-1967]es_ES
dc.contributor.orcidMartins, Viviana [0000-0001-9218-6524]es_ES
dc.contributor.orcidAguilar, Emmanuel [0000-0001-8222-3168]es_ES
dc.contributor.orcidCanto, Tomás [0000-0001-8017-6345]es_ES
dc.contributor.orcidTenllado, Francisco [0000-0002-5349-7642]es_ES
dc.contributor.orcidDias, Alberto C. P. [0000-0003-3641-3248]es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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