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Title

Novel intracellular antibiotic delivery system against Staphylococcus aureus: cloxacillin-loaded poly(d,l-lactide-co-glycolide) acid nanoparticles

AuthorsLacoma, Alicia; Usón, Laura; Mendoza, Gracia; Sebastian, Victor; Garcia-Garcia, Esther; Muriel-Moreno, Beatriz; Domínguez, Jose; Arruebo, Manuel; Prat, Cristina
KeywordsAntimicrobialdrug delivery
Staphylococcus aureus
Intracellular infection
Nanoparticle
Issue Date2020
PublisherFuture Medicine
CitationNanomedicine 15(12): 1189–1203 (2020)
Abstract[Aim]: First, to compare in vitro minimum inhibitory concentrations (MIC) of free cloxacillin and cloxacillin-containing nanoparticles (NP) against methicillin-susceptible (MSSA) and resistant Staphylococcus aureus (MRSA) and second, to assess NP antimicrobial activity against intracellular S. aureus.
[Methods]: Poly(d,l-lactide-co-glycolide) acid (PLGA)-NP were loaded with cloxacillin and physico-chemically characterized. MICs were determined for reference strains Newman-(MSSA) and USA300-(MRSA). Murine alveolar macrophages were infected, and bacterial intracellular survival was assessed after incubating with free-cloxacillin or PLGA-cloxacillin-NP.
[Results and conclusion]: For both isolates, MICs for antibiotic-loaded-NP were lower than those obtained with free cloxacillin, indicating that the drug encapsulation improves antimicrobial activity. A sustained antibiotic release was demonstrated when using the PLGA-cloxacillin-NP. When considering the lowest concentrations, the use of drug-loaded NP enabled a higher reduction of intracellular bacterial load.
Publisher version (URL)https://doi.org/10.2217/nnm-2019-0371
URIhttp://hdl.handle.net/10261/219043
DOI10.2217/nnm-2019-0371
ISSN1743-5889
E-ISSN1748-6963
Appears in Collections:(ICMA) Artículos
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