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dc.contributor.authorSánchez-Casanova, Silviaes_ES
dc.contributor.authorMartín-Saavedra, Franciscoes_ES
dc.contributor.authorEscudero-Duch, Claraes_ES
dc.contributor.authorFalguera Uceda, Maria I.es_ES
dc.contributor.authorPrieto, Martines_ES
dc.contributor.authorArruebo, Manueles_ES
dc.contributor.authorAcebo, Palomaes_ES
dc.contributor.authorFabiilli, Mario L.es_ES
dc.contributor.authorFranceschi, Renny T.es_ES
dc.contributor.authorVilaboa, Nuriaes_ES
dc.date.accessioned2020-09-02T10:58:16Z-
dc.date.available2020-09-02T10:58:16Z-
dc.date.issued2020-
dc.identifier.citationBiomaterials 241: 119909 (2020)es_ES
dc.identifier.issn0142-9612-
dc.identifier.urihttp://hdl.handle.net/10261/219007-
dc.description.abstractAchievement of spatiotemporal control of growth factors production remains a main goal in tissue engineering. In the present work, we combined inducible transgene expression and near infrared (NIR)-responsive hydrogels technologies to develop a therapeutic platform for bone regeneration. A heat-activated and dimerizer-dependent transgene expression system was incorporated into mesenchymal stem cells to conditionally control the production of bone morphogenetic protein 2 (BMP-2). Genetically engineered cells were entrapped in hydrogels based on fibrin and plasmonic gold nanoparticles that transduced incident energy of an NIR laser into heat. In the presence of dimerizer, photoinduced mild hyperthermia induced the release of bioactive BMP-2 from NIR-responsive cell constructs. A critical size bone defect, created in calvaria of immunocompetent mice, was filled with NIR-responsive hydrogels entrapping cells that expressed BMP-2 under the control of the heat-activated and dimerizer-dependent gene circuit. In animals that were treated with dimerizer, NIR irradiation of implants induced BMP-2 production in the bone lesion. Induction of NIR-responsive cell constructs conditionally expressing BMP-2 in bone defects resulted in the formation of new mineralized tissue, thus indicating the therapeutic potential of the technological platform.es_ES
dc.description.sponsorshipThis work was supported by grant PI15/01118 from ISCIII-Fondos FEDER, Ministerio de Economía y Competitividad (MINECO), Spain, grant RTI2018-095159-B-I00 from Ministerio de Ciencia, Innovación y Universidades (MICINN), Spain, grant Roche-IdiPAZ from the intramural funding program of Foundation for Biomedical Research of Hospital Universitario La Paz-IdiPAZ, grant ERC-2013-CoG-614715 (NANOHEDONISM) from ERC Consolidator Grant program, grant R21AR072336 from NIH, United States, and by HSF Pharmaceuticals S.A. C.E-D. was the recipient of predoctoral grant FI14/00447 from ISCIII-Fondos FEDER, Ministerio de Economía y Competitividad (MINECO). N.V. is supported by Program I2 from Comunidad Autónoma de Madrid.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMICIU/ICTI2017-2020/RTI2018-095159-B-I00es_ES
dc.relationRTI2018-095159-B-I00/AEI/10.13039/501100011033es_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/614715es_ES
dc.rightsclosedAccesses_ES
dc.subjectNear-infraredes_ES
dc.subjectHydrogeles_ES
dc.subjectGene therapyes_ES
dc.subjectBone morphogenetic protein 2es_ES
dc.titleLocal delivery of bone morphogenetic protein-2 from near infrared-responsive hydrogels for bone tissue regenerationes_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2020.119909-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.biomaterials.2020.119909es_ES
dc.contributor.funderInstituto de Salud Carlos IIIes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderAgencia Estatal de Investigación (España)es_ES
dc.contributor.funderEuropean Research Counciles_ES
dc.contributor.funderInstituto de Investigación Hospital Universitario La Pazes_ES
dc.contributor.funderNational Institutes of Health (US)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderRochees_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000781es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004587es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100000002es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100004337es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011033es_ES
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