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http://hdl.handle.net/10261/218928
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dc.contributor.author | Sun, Junjun | es_ES |
dc.contributor.author | Chen, Cuiying | es_ES |
dc.contributor.author | You, Cuihong | es_ES |
dc.contributor.author | Liu, Yang | es_ES |
dc.contributor.author | Ma, Hong Yu | es_ES |
dc.contributor.author | Monroig, Óscar | es_ES |
dc.contributor.author | Tocher, Douglas R. | es_ES |
dc.contributor.author | Wang, Shuqi | es_ES |
dc.contributor.author | Li, Yuanyou | es_ES |
dc.date.accessioned | 2020-09-01T09:04:14Z | - |
dc.date.available | 2020-09-01T09:04:14Z | - |
dc.date.issued | 2020-05-16 | - |
dc.identifier.citation | Marine Biotechnology 22: 475-487(2020) | es_ES |
dc.identifier.issn | 1436-2228 | - |
dc.identifier.uri | http://hdl.handle.net/10261/218928 | - |
dc.description.abstract | Post-transcriptional regulatory mechanisms play important roles in the regulation of long-chain (≥ C20) polyunsaturated fatty acid (LC-PUFA) biosynthesis. Here, we address a potentially important role of the miR-15/16 cluster in the regulation of LC-PUFA biosynthesis in rabbitfish Siganus canaliculatus. In rabbitfish, miR-15 and miR-16 were both highly responsive to fatty acids affecting LC-PUFA biosynthesis and displayed a similar expression pattern in a range of rabbitfish tissues. A common potential binding site for miR-15 and miR-16 was predicted in the 3′UTR of peroxisome proliferator-activated receptor gamma (pparγ), an inhibitor of LC-PUFA biosynthesis in rabbitfish, and luciferase reporter assays revealed that pparγ was a potential target of miR-15/16 cluster. In vitro individual or co-overexpression of miR-15 and miR-16 in rabbitfish hepatocyte line (SCHL) inhibited both mRNA and protein levels of Pparγ, and increased the mRNA levels of Δ6Δ5 fads2, Δ4 fads2, and elovl5, key enzymes of LC-PUFA biosynthesis. Inhibition of pparγ was more pronounced with co-overexpression of miR-15 and miR-16 than with individual overexpression in SCHL. Knockdown of miR-15/16 cluster gave opposite results, and increased mRNA levels of LC-PUFA biosynthesis enzymes were observed after knockdown of pparγ. Furthermore, miR-15/16 cluster overexpression significantly increased the contents of 22:6n-3, 20:4n-6 and total LC-PUFA in SCHL with higher 18:4n-3/18:3n-3 and 22:6n-3/22:5n-3 ratio. These suggested that miR-15 and miR-16 as a miRNA cluster together enhanced LC-PUFA biosynthesis by targeting pparγ in rabbitfish. This is the first report of the participation of miR-15/16 cluster in LC-PUFA biosynthesis in vertebrates. | es_ES |
dc.description.sponsorship | This work was financially supported by the National Key R&D Program of China (2018YFD0900400), National Natural Science Foundation of China (No. 31873040 & No. 31702357), Natural Science Foundation of Guangdong Province (2018A030313910), China Agriculture Research System (CARS-47), Innovation and Strong School Projects in Guangdong Province (2016KTSCX037), STU Scientific Research Foundation for Talents (NTF19019), Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding (2017B030314058), and Natural Science Foundation of Guangdong Province (2020A1515011252). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.relation.isversionof | Postprint | es_ES |
dc.rights | openAccess | en_EN |
dc.subject | miR-15/16 cluste | es_ES |
dc.subject | pparγ | es_ES |
dc.subject | LC-PUFA biosynthesis | es_ES |
dc.subject | Rabbitfish Siganus canaliculatus | es_ES |
dc.title | The miR-15/16 Cluster Is Involved in the Regulation of Vertebrate LC-PUFA Biosynthesis by Targeting pparγ as Demonostrated in Rabbitfish Siganus canaliculatus | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1007/s10126-020-09969-0 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1007/s10126-020-09969-0 | es_ES |
dc.identifier.e-issn | 1436-2236 | - |
dc.embargo.terms | 2021-05-16 | es_ES |
dc.contributor.funder | National Key Research and Development Program (China) | es_ES |
dc.contributor.funder | National Natural Science Foundation of China | es_ES |
dc.contributor.funder | Natural Science Foundation of Guangdong Province | es_ES |
dc.contributor.funder | Agriculture Research System of China | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100010203 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100001809 | es_ES |
dc.contributor.orcid | Monroig, Óscar [0000-0001-8712-0440] | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
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