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Ultrasensitive detection of alpha-synuclein oligomer using a PolyD-glucosamine/gold nanoparticle/carbon-based nanomaterials modified electrochemical immunosensor in human plasma

AuthorsTao, Dan; Gu, Yingying; Song, Shizhen; Nguyen, Emily P.; Cheng, Jing; Yuan, Qiong; Pan, Hongzhi; Jaffrezic-Renault, Nicole; Guo, Zhenzhong
Issue Date2020
CitationMicrochemical Journal 158: 105195 (2020)
AbstractTo discover the early stage Parkinson’s disease (PD) patients and provide treatment ideas, an ultrasensitive poly (D-glucosamine)/gold nanoparticles/ multi-walled carbon nanotubes/reduced graphene oxide (PDG/AuNPs/MWCNTs/rGO) modified immunosensor was proposed to detect alpha-synuclein oligomer (α-syno), which is the early biomarker in PD. As signal amplification materials, the PDG/AuNPs/ MWCNTs/rGO nanocomplex was immobilized on the glassy carbon electrode surface, and anti-α-syno antibody that specifically recognizes and binds α-syno is then grafted. The immunosensor revealed a linear response between 0.05 fM and 500.00 fM, using square wave voltammetry (SWV), and a detection limit of 0.03fM was found. The immunosensor with excellent sensitivity, selectivity and stability has been applied to the analysis of α-syno in human plasma samples.
Publisher version (URL)https://doi.org/10.1016/j.microc.2020.105195
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