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dc.contributor.authorPeralta-Sánchez, Juan M-
dc.contributor.authorTravaglia, C.N.-
dc.contributor.authorRomero-Puertas, María C.-
dc.contributor.authorFurlan, A.-
dc.contributor.authorCastro, S.-
dc.contributor.authorBianucci, E.-
dc.identifierdoi: 10.1016/j.chemosphere.2020.127410-
dc.identifierissn: 1879-1298-
dc.identifier.citationChemosphere 259: no. 127410 (2020)-
dc.description.abstractArsenic (As) can be present naturally in groundwater from peanut fields, constituting a serious problem, as roots can accumulate and mobilize the metalloid to their edible parts. Understanding the redox changes in the legume exposed to As may help to detect potential risks to human health and recognize tolerance mechanisms. Thirty-days old peanut plants inoculated with Bradyrhizobium sp. strains (SEMIA6144 or C-145) were exposed to a realistic arsenate concentration, in order to unravel the redox response and characterize the oxidative stress indexes. Thus, root anatomy, reactive oxygen species detection by fluorescence microscopy and, ROS histochemical staining along with the NADPH oxidase activity were analyzed. Besides, photosynthetic pigments and damage to lipids and proteins were determined as oxidative stress indicators. Results showed that at 3 μM As, the cross-section areas of peanut roots were augmented; NADPH oxidase activity was significantly increased and O˙¯and HO accumulated in leaves and roots. Likewise, an increase in the lipid peroxidation and protein carbonyls was also observed throughout the plant regardless the inoculated strain, while chlorophylls and carotenes were increased only in those inoculated with Bradyrhizobium sp. C-145. Interestingly, the oxidative burst, mainly induced by the NADPH oxidase activity, and the consequent oxidative stress was strain-dependent and organ-differential. Additionally, As modifies the root anatomy, acting as a possibly first defense mechanism against the metalloid entry. All these findings allowed us to conclude that the redox response of peanut is conditioned by the rhizobial strain, which contributes to the importance of effectively formulating bioinoculants for this crop.-
dc.description.sponsorshipThis research was financially supported by Secretaría de Ciencia y Tecnología-Universidad Nacional de Río Cuarto (SECYT-UNRC); Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Ministry of Science, Innovation and Universities together with the European Regional Development Fund (MCIU/AEI/ERDF; PGC2018-098372-B-100). JMP is a CONICET scholarship. CT, EB and AF are members of the research career from CONICET. MCRP is research scientist at EEZ-CSIC. SC is a researcher-teacher at UNRC. JMP thanks the Programa de Movilidad entre Instituciones Asociadas a la Asociación Universitaria Iberoamericana de Postgrado (AUIP, 2017) for supporting a short-term scholarship in EEZ-CSIC and Universidad de Granada (Spain). The authors are grateful to Eliana Molina-Moya and Dr. Adela Olmedilla for their technical assistance.-
dc.subjectArachis hypogaea L.-
dc.subjectOxidative stress-
dc.subjectReactive oxygen species-
dc.titleUnraveling the impact of arsenic on the redox response of peanut plants inoculated with two different Bradyrhizobium sp. strains-
dc.contributor.funderAgencia Nacional de Promoción Científica y Tecnológica (Argentina)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderUniversidad Nacional de Río Cuarto (Argentina)-
dc.contributor.funderUniversidad de Granada-
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)-
dc.contributor.funderConsejo Nacional de Investigaciones Científicas y Técnicas (Argentina)-
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