English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/218822
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


The use of isothermal titration calorimetry to unravel chemotactic signalling mechanisms

AuthorsMatilla, Miguel A.; Martín-Mora, David; Krell, Tino
Issue Date2020
PublisherBlackwell Publishing
CitationEnvironmental Microbiology 22(8): 3005–3019 (2020)
AbstractChemotaxis is based on the action of chemosensory pathways and is typically initiated by the recognition of chemoeffectors at chemoreceptor ligand-binding domains (LBD). Chemosensory signalling is highly complex; aspect that is not only reflected in the intricate interaction between many signalling proteins but also in the fact that bacteria frequently possess multiple chemosensory pathways and often a large number of chemoreceptors, which are mostly of unknown function. We review here the usefulness of isothermal titration calorimetry (ITC) to study this complexity. ITC is the gold standard for studying binding processes due to its precision and sensitivity, as well as its capability to determine simultaneously the association equilibrium constant, enthalpy change and stoichiometry of binding. There is now evidence that members of all major LBD families can be produced as individual recombinant proteins that maintain their ligand-binding properties. High-throughput screening of these proteins using thermal shift assays offer interesting initial information on chemoreceptor ligands, providing the basis for microcalorimetric analyses and microbiological experimentation. ITC has permitted the identification and characterization of many chemoreceptors with novel specificities. This ITC-based approach can also be used to identify signal molecules that stimulate members of other families of sensor proteins.
Publisher version (URL)http://dx.doi.org/10.1111/1462-2920.15035
Identifiersdoi: 10.1111/1462-2920.15035
issn: 1462-2920
Appears in Collections:(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
2020_Matilla_EM_OA.pdf1,09 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.