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Comparative analysis of β-carotene production by mucor circinelloides strains CBS 277.49 and WJ11 under light and dark conditions

AuthorsNaz, T.; Nosheen, S.; Li, S.; Nazir, Y.; Mustafa, K.; Liu, Q.; Garre, Victoriano; Song, Y.
Mucor circinelloides strains
Mevalonate pathway
Transcriptional levels
Issue Date16-Jan-2020
PublisherMultidisciplinary Digital Publishing Institute
CitationMetabolites 10: 38 (2020)
AbstractCarotenoids are natural potent antioxidants and free radical scavengers which are able to modulate the pathogenesis of some cancers and heart diseases in human, indicating their importance in being provided through the diet. Mucor circinelloides accumulates β-carotene as the main carotenoid compound and has been used as a model organism in carotenogenic studies. In the present study, the potential of two M. circinelloides strains to accumulate β-carotene was investigated under light and dark conditions. The results, which were quantitated by HPLC, showed that CBS 277.49 accumulated higher pigment in comparison to WJ11 under both conditions. Continuous illumination triggered the pigment accumulation up to 2.7-fold in strain CBS 277.49 and 2.2-fold in strain WJ11 in comparison to dark. The mRNA analysis of the four key genes involved in isoprenoid pathway by RT-qPCR showed higher transcriptional levels in CBS 277.49 as compared to WJ11, indicating that the pigment production metabolic machinery is more active in CBS 277.49 strain. A new scope for further research was established by this work for improved β-carotene production in the high producing strain CBS 277.49.
Description14 pags., 5 figs., 2 tabs.
Publisher version (URL)http://dx.doi.org/10.3390/metabo10010038
Identifiersdoi: 10.3390/metabo10010038
issn: 2218-1989
Appears in Collections:(IQFR) Artículos
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