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Title

High Hydrostatic Pressure Assisted by Celluclast® Releases Oligosaccharides from Apple By-Product

AuthorsPeña Armada, Rocío de la; Villanueva Suárez, M. José; Rupérez Antón, Pilar ; Mateos-Aparicio, Inmaculada
KeywordsApple by-product
Valorisation
Green technology
HHP
Cellulase
Dietary fibre
Polysaccharides
Oligosaccharides
Prebiotics
Issue Date2020
PublisherMultidisciplinary Digital Publishing Institute
CitationFoods 9(8): 1058 (2020)
AbstractA novel and green procedure consisting of high hydrostatic pressure (HHP) aided by a commercial cellulase (Celluclast®) has been applied to valorise the apple by-product, a valuable source of dietary fibre but mainly composed by insoluble fibre. Optimal conditions for solubilisation of dietary fibre were first determined at atmospheric pressure as 2% (w/v) of substrate concentration and 20 Endo-Glucanase Units of cellulase. Monitoring of polysaccharides and oligosaccharides released from apple by-product was carried out by means of a newly validated HPLC method with refractive index detector. A synergistic effect was observed when the combined HHP plus cellulase treatment was used. Thus, the application of 200 MPa at 50 °C for 15 min enabled a significant increase in the release of water-soluble polysaccharides (1.8-fold) and oligosaccharides (3.8-fold), as well as a considerable decrease in the time required (up to 120-fold), compared to control at 0.1 MPa. Therefore, this technology could be a promising alternative approach to transform an industrial by-product into a novel rich-in-oligosaccharide food ingredient and a step forward into shaping the world of prebiotics.
Publisher version (URL)https://doi.org/10.3390/foods9081058
URIhttp://hdl.handle.net/10261/218429
DOI10.3390/foods9081058
E-ISSN2304-8158
Appears in Collections:(ICTAN) Artículos
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