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dc.contributor.authorLu, Yameng-
dc.contributor.authorOcaña-Pallarès, Eduard-
dc.contributor.authorLópez-Escardó, David-
dc.contributor.authorDennis, Stuart R.-
dc.contributor.authorMonaghan, Michael T.-
dc.contributor.authorRuiz-Trillo, Iñaki-
dc.contributor.authorSpaak, Piet-
dc.contributor.authorWolinska, Justyna-
dc.date.accessioned2020-08-21T09:40:10Z-
dc.date.available2020-08-21T09:40:10Z-
dc.date.issued2020-10-
dc.identifierissn: 1055-7903-
dc.identifiere-issn: 1095-9513-
dc.identifier.citationMolecular Phylogenetics and Evolution 151: 106891 (2020)-
dc.identifier.otherCEX2019-000928-S-
dc.identifier.urihttp://hdl.handle.net/10261/218367-
dc.description8 pages, 3 figures, 1 table, supplementary material https://doi.org/10.1016/j.ympev.2020.106891.-- The genome data have been submitted to SRA at NCBI under the Accession no. PRJNA631182. The primary sequences have been submitted to NCBI under the Accession no. SRX8386394 - SRX8386397-
dc.description.abstractCaullerya mesnili is a common and virulent parasite of the water flea, Daphnia. It was classified within the Haplosporidia (Rhizaria) for over a century. However, a recent molecular phylogeny based on the 18S rRNA gene suggested it belonged to the Ichthyosporea, a class of protists closely related to animals within the Opisthokonta clade. The exact phylogenetic position of C. mesnili remained uncertain because it appeared in the 18S rRNA tree with a very long branch and separated from all other taxa, suggesting that its position could be artifactual. A better understanding of its phylogenetic position has been constrained by a lack of molecular markers and the difficulty of obtaining a suitable quantity and quality of DNA from in vitro cultures, as this intracellular parasite cannot be cultured without its host. We isolated and collected spores of C. mesnili and sequenced genomic libraries. Phylogenetic analyses of a newly generated multi-protein data set (22 proteins, 4998 amino acids) and of sequences from the 18S rRNA gene both placed C. mesnili within the Ichthyophonida sub-clade of Ichthyosporea, as sister-taxon to Abeoforma whisleri and Pirum gemmata. Our study highlights the utility of metagenomic approaches for obtaining genomic information from intracellular parasites and for more accurate phylogenetic placement in evolutionary studies-
dc.description.sponsorshipThis work was supported by a joint “lead agency” grant from the German Science Foundation (WO 1587/6-1 to JW) and Swiss National Science Foundation (310030 L 166628 to PS). We also acknowledge support from a grant (BFU2014-57779-P and BFU2017-90114-P) from the Ministerio de Economía y Competitividad (MINECO), Agencia Estatal de Investigación (AEI), and Fondo Europeo de Desarrollo Regional (FEDER) to IRT. EOP was supported by a pre-doctoral FPI grant from MINECO-
dc.description.sponsorshipWith the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI)-
dc.languageeng-
dc.publisherElsevier-
dc.publisherAcademic Press-
dc.relationMINECO/ICTI2013-2016/BFU2014-57779-P-
dc.relationMICIU/ICTI2017-2020/BFU2017-90114-P-
dc.rightsclosedAccess-
dc.subjectEvolution-
dc.subjectMetagenomic-
dc.subjectMolecular systematics-
dc.subjectMulti-protein phylogeny-
dc.subjectProtists-
dc.subjectPhylogenomics-
dc.subject18S rRNA gene-
dc.titleRevisiting the phylogenetic position of Caullerya mesnili (Ichthyosporea), a common Daphnia parasite, based on 22 protein-coding genes-
dc.typeartículo-
dc.identifier.doihttp://dx.doi.org/10.1016/j.ympev.2020.106891-
dc.relation.publisherversionhttps://doi.org/10.1016/j.ympev.2020.106891-
dc.date.updated2020-08-21T09:40:11Z-
dc.contributor.funderGerman Research Foundation-
dc.contributor.funderSwiss National Science Foundation-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)-
dc.contributor.funderAgencia Estatal de Investigación (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderAgencia Estatal de Investigación (España)-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011033es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001659es_ES
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