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Title

Revisiting the phylogenetic position of Caullerya mesnili (Ichthyosporea), a common Daphnia parasite, based on 22 protein-coding genes

AuthorsLu, Yameng; Ocaña-Pallarès, Eduard; López-Escardó, David CSIC ORCID ; Dennis, Stuart R.; Monaghan, Michael T.; Ruiz-Trillo, Iñaki CSIC ORCID ; Spaak, Piet; Wolinska, Justyna
KeywordsEvolution
Metagenomic
Molecular systematics
Multi-protein phylogeny
Protists
Phylogenomics
18S rRNA gene
Issue DateOct-2020
PublisherElsevier
Academic Press
CitationMolecular Phylogenetics and Evolution 151: 106891 (2020)
AbstractCaullerya mesnili is a common and virulent parasite of the water flea, Daphnia. It was classified within the Haplosporidia (Rhizaria) for over a century. However, a recent molecular phylogeny based on the 18S rRNA gene suggested it belonged to the Ichthyosporea, a class of protists closely related to animals within the Opisthokonta clade. The exact phylogenetic position of C. mesnili remained uncertain because it appeared in the 18S rRNA tree with a very long branch and separated from all other taxa, suggesting that its position could be artifactual. A better understanding of its phylogenetic position has been constrained by a lack of molecular markers and the difficulty of obtaining a suitable quantity and quality of DNA from in vitro cultures, as this intracellular parasite cannot be cultured without its host. We isolated and collected spores of C. mesnili and sequenced genomic libraries. Phylogenetic analyses of a newly generated multi-protein data set (22 proteins, 4998 amino acids) and of sequences from the 18S rRNA gene both placed C. mesnili within the Ichthyophonida sub-clade of Ichthyosporea, as sister-taxon to Abeoforma whisleri and Pirum gemmata. Our study highlights the utility of metagenomic approaches for obtaining genomic information from intracellular parasites and for more accurate phylogenetic placement in evolutionary studies
Description8 pages, 3 figures, 1 table, supplementary material https://doi.org/10.1016/j.ympev.2020.106891.-- The genome data have been submitted to SRA at NCBI under the Accession no. PRJNA631182. The primary sequences have been submitted to NCBI under the Accession no. SRX8386394 - SRX8386397
Publisher version (URL)https://doi.org/10.1016/j.ympev.2020.106891
URIhttp://hdl.handle.net/10261/218367
DOI10.1016/j.ympev.2020.106891
Identifiersissn: 1055-7903
e-issn: 1095-9513
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