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Title

Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater

AuthorsRusiñol, Marta; Martínez-Puchol, Sandra; Forés, Eva; Itarte, Marta; Girones, Rosina; Bofill-Mas, Sílvia
KeywordsConcentration methods
Enveloped virus
SARS-CoV-2
Recovery efficiency
Surrogate virus
Issue Date19-Aug-2020
PublisherElsevier BV
CitationCurrent Opinion in Environmental Science and Health (2020)
AbstractSince the novel SARS-CoV-2 was detected in faeces, environmental researchers have been using centrifugal ultrafiltration, polyethylene glycol precipitation and aluminium hydroxide flocculation to describe its presence in wastewater samples. High recoveries (up to 65%) are described with electronegative filtration when using surrogate viruses, but few literature reports recovery efficiencies using accurate quantification of enveloped viruses. Considering that every single virus will have a different behaviour during viral concentration, it is recommended to use an enveloped virus, and if possible, a betacoronaviruses as murine hepatitis virus (MHV), as a surrogate. In this review we show new data from a new available technology that provides a quick ultrafiltration protocol for SARS-CoV-2. Wastewater surveillance is an efficient system for the evaluation of the relative prevalence of SARS-CoV-2 infections in a community, and there is the need of using reliable concentration methods for an accurate and sensitive quantification of the virus in water.
Publisher version (URL)https://doi.org/10.1016/j.coesh.2020.08.002
URIhttp://hdl.handle.net/10261/218352
DOIhttp://dx.doi.org/10.1016/j.coesh.2020.08.002
ISSN2468-5844
Appears in Collections:(IDAEA) Artículos
(VICYT) Colección Especial COVID-19
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