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Title

Studies of the secretory machinery dynamics by total internal reflection fluorescence microscopy in bovine adrenal chromaffin cells

AuthorsVillanueva, José; Gimenez-Molina, Yolanda; Gutiérrez, Luis M.
KeywordsTIRFM
Evanescent field
Exocytotic events
SNARE proteins
Chromaffin granules
Chromaffin cells
Issue Date2019
PublisherSpringer Nature
CitationSNAREs. Methods and Protocols: 379-389 (2019)
SeriesMethods in Molecular Biology 1860
AbstractCultured bovine chromaffin cells have been tested as a successful neuroendocrine model to study the secretory process. Changes in the dynamics of the secretory vesicles and the exocytotic machinery microdomains could be studied in control and stimulated conditions using appropriate molecular tools such as fluorescent SNARE protein expression or fluorochrome vesicular labeling in these neuroendocrine cells. Since most of these changes occur in or near the plasma membrane, the use of the total internal reflection fluorescent microscopy (TIRFM) and the implement of particle motion analysis could be essential tools to study the structural and dynamic changes of secretory machinery related with its function in this exocytotic cell model.
Publisher version (URL)https://doi.org/10.1007/978-1-4939-8760-3_25
URIhttp://hdl.handle.net/10261/218319
DOIhttp://dx.doi.org/10.1007/978-1-4939-8760-3_25
ISBN978-1-4939-8759-7
Appears in Collections:(IN) Libros y partes de libros
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