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Characterization of TRPC channels in a heterologous system using calcium imaging and the patch-clamp technique

AuthorsPeña, Elvira de la; Gomis, Ana
KeywordsIntracellular calcium recordings
Patch clamp
Osmomechanical stimulation
TRP channels
Issue Date2019
PublisherSpringer Nature
CitationTRP Channels. Methods and Protocols: 83-97 (2019)
SeriesMethods in Molecular Biology 1987
AbstractThe family of transient receptor potential (TRPs) channels contains 28 mammalian members, each a unique cellular sensor that responds to a wide variety of external and internal signals. TRP channels are expressed by most mammalian cells, where they are involved in many different physiological functions. Canonical TRP channels (TRPCs) form a family of nonselective cationic channels, although with greater selectivity for Ca2+. This family is made up of seven members (TRPC1-7), all of which contain a TRP box in the carboxyl terminal and 3-4 ankyrin repeats in the amino terminal. While these channels share some similar properties, they display diverse gating mechanisms and are involved in different signaling pathways (Gees M et al., Compr Physiol, 2012). The activation or inhibition of these channels has been studied using different approaches and techniques. Here, we characterize the activation of the TRPC5 channel expressed in a heterologous system, using calcium imaging and the patch-clamp technique in whole-cell configuration.
Publisher version (URL)https://doi.org/10.1007/978-1-4939-9446-5_6
Appears in Collections:(IN) Libros y partes de libros
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