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Title

Chestnut, European (Castanea sativa)

AuthorsCorredoira, Elena ; Valladares, Silvia; Viéitez Martín, Ana María ; Ballester, Antonio
KeywordsAgrobacterium tumefaciens
Castanea sativa
Forest biotechnology
Genetic transformation
gfp
Somatic embryogenesis
Thaumatin-like protein
Issue Date2015
PublisherSpringer Nature
CitationAgrobacterium Protocols (14): 163-176 (2015)
SeriesMethods in Molecular Biology
1224
AbstractDevelopment of a system for direct transfer of antifungal candidate genes into European chestnut (Castanea sativa) would provide an alternative approach to conventional breeding for production of chestnut trees that are tolerant to ink disease caused by Phytophthora spp. Overexpression of genes encoding PR proteins (such as thaumatin-like proteins), which display antifungal activity, may represent an important advance in control of the disease. We have used a chestnut thaumatin-like protein gene (CsTL1) isolated from European chestnut cotyledons and have achieved overexpression of the gene in chestnut somatic embryogenic lines used as target material. We have also acclimatized the transgenic plants and grown them on in the greenhouse. Here, we describe the various steps of the process, from the induction of somatic embryogenesis to the production of transgenic plants.
Publisher version (URL)https://doi.org/10.1007/978-1-4939-1658-0_14
URIhttp://hdl.handle.net/10261/218273
DOI10.1007/978-1-4939-1658-0_14
ISBN978-1-4939-1657-3
Appears in Collections:(IIAG) Libros y partes de libros
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