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Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome

AuthorsCastro López, Antonio Jesús; Lima Cabello, Elena; Alché Ramírez, Juan de Dios
KeywordsExtra virgin olive oil (EVOO)
Olea europaea
seed storage proteins (SSP)
Issue Date2020
CitationFood Chemistry: X 7: 100099 (2020)
AbstractProteins are minor components of extra virgin olive oil (EVOO), but the nature of the olive oil proteome is still elusive. In this paper, we have uncovered the EVOO proteome for the first time. Seed storage proteins of globulin-type were identified as the most abundant proteins in EVOO, which also contains an active 13-lipoxygenase and several potential allergenic proteins, including the “panallergen” profilin. We validated our proteomic data by Western blotting and enzyme activity assays. Our data also demonstrated that the seed is the main source of proteins in EVOO, while the contribution of the pulp is uncertain and needs further verification. The impact of EVOO proteins on its stability and quality, and on human health is discussed.
Publisher version (URL)http://dx.doi.org/10.1016/j.fochx.2020.100099
Identifiersdoi: 10.1016/j.fochx.2020.100099
issn: 2590-1575
Appears in Collections:(EEZ) Artículos
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