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Title

Deregulated High Affinity Copper Transport Alters Iron Homeostasis in Arabidopsis

AuthorsPerea García, Ana ; Andrés Bordería, Amparo; Vera Sirera, Francisco ; Pérez Amador, Miguel Ángel; Puig, Sergi ; Peñarrubia, Lola
KeywordsArabidopsis thaliana
Copper uptake
High affinity copper importer 1
Iron homeostasis
Metal interactions
Metal mobilization
Issue Date23-Jul-2020
PublisherFrontiers Media
CitationFrontiers in Plant Science 11: 1106 (2020)
AbstractThe present work describes the effects on iron homeostasis when copper transport was deregulated in Arabidopsis thaliana by overexpressing high affinity copper transporters COPT1 and COPT3 (COPTOE). A genome-wide analysis conducted on COPT1OE plants, highlighted that iron homeostasis gene expression was affected under both copper deficiency and excess. Among the altered genes were those encoding the iron uptake machinery and their transcriptional regulators. Subsequently, COPTOE seedlings contained less iron and were more sensitive than controls to iron deficiency. The deregulation of copper (I) uptake hindered the transcriptional activation of the subgroup Ib of basic helix-loop-helix (bHLH-Ib) factors under copper deficiency. Oppositely, copper excess inhibited the expression of the master regulator FIT but activated bHLH-Ib expression in COPTOE plants, in both cases leading to the lack of an adequate iron uptake response. As copper increased in the media, iron (III) was accumulated in roots, and the ratio iron (III)/iron (II) was increased in COPTOE plants. Thus, iron (III) overloading in COPTOE roots inhibited local iron deficiency responses, aimed to metal uptake from soil, leading to a general lower iron content in the COPTOE seedlings. These results emphasized the importance of appropriate spatiotemporal copper uptake for iron homeostasis under non-optimal copper supply. The understanding of the role of copper uptake in iron metabolism could be applied for increasing crops resistance to iron deficiency.
Publisher version (URL)https://doi.org/10.3389/fpls.2020.01106
URIhttp://hdl.handle.net/10261/217904
DOI10.3389/fpls.2020.01106
E-ISSN1664-462X
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