English
español
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/217878
Share/Impact:
Statistics |
![]() ![]() ![]() |
|
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |||
|
Title: | KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain |
Authors: | Lipinski, Michal; Muñoz-Viana, Rafael; Blanco, Beatriz del; Marquez-Galera, Angel; Medrano-Relinque, Juan; Caramés, José M.; Szczepankiewicz, Andrzej A.; Fernandez-Albert, Jordi; Navarrón, Carmen M.; Olivares, Román; Wilczyński, Grzegorz M.; Canals Gamoneda, Santiago; López-Atalaya, José P.; Barco, Ángel |
Issue Date: | 2020 |
Publisher: | Springer Nature |
Citation: | Nature Communications 11: 2588 (2020) |
Abstract: | The lysine acetyltransferases type 3 (KAT3) family members CBP and p300 are important transcriptional co-activators, but their specific functions in adult post-mitotic neurons remain unclear. Here, we show that the combined elimination of both proteins in forebrain excitatory neurons of adult mice resulted in a rapidly progressing neurological phenotype associated with severe ataxia, dendritic retraction and reduced electrical activity. At the molecular level, we observed the downregulation of neuronal genes, as well as decreased H3K27 acetylation and pro-neural transcription factor binding at the promoters and enhancers of canonical neuronal genes. The combined deletion of CBP and p300 in hippocampal neurons resulted in the rapid loss of neuronal molecular identity without de- or transdifferentiation. Restoring CBP expression or lysine acetylation rescued neuronal-specific transcription in cultured neurons. Together, these experiments show that KAT3 proteins maintain the excitatory neuron identity through the regulation of histone acetylation at cell type-specific promoter and enhancer regions. |
Publisher version (URL): | https://doi.org/10.1038/s41467-020-16246-0 |
URI: | http://hdl.handle.net/10261/217878 |
DOI: | http://dx.doi.org/10.1038/s41467-020-16246-0 |
E-ISSN: | 2041-1723 |
Appears in Collections: | (IN) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
kat3brain.pdf | 6,65 MB | Adobe PDF | ![]() View/Open |
Show full item record
Review this work
Review this work
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.