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Title

Immediate and deferred epigenomic signatures of in vivo neuronal activation in mouse hippocampus

AuthorsFernandez-Albert, Jordi; Lipinski, Michal; Lopez-Cascales, María T.; Jordan Rowley, M.; Martin-Gonzalez, Ana M.; Blanco, Beatriz del; Corces, Victor G.; Barco, Ángel
Issue Date2019
PublisherSpringer Nature
CitationNature Neuroscience 22: 1718–1730 (2019)
AbstractActivity-driven transcription plays an important role in many brain processes, including those underlying memory and epilepsy. Here we combine genetic tagging of nuclei and ribosomes with RNA sequencing, chromatin immunoprecipitation with sequencing, assay for transposase-accessible chromatin using sequencing and Hi-C to investigate transcriptional and chromatin changes occurring in mouse hippocampal excitatory neurons at different time points after synchronous activation during seizure and sparse activation by novel context exploration. The transcriptional burst is associated with an increase in chromatin accessibility of activity-regulated genes and enhancers, de novo binding of activity-regulated transcription factors, augmented promoter–enhancer interactions and the formation of gene loops that bring together the transcription start site and transcription termination site of induced genes and may sustain the fast reloading of RNA polymerase complexes. Some chromatin occupancy changes and interactions, particularly those driven by AP1, remain long after neuronal activation and could underlie the changes in neuronal responsiveness and circuit connectivity observed in these neuroplasticity paradigms, perhaps thereby contributing to metaplasticity in the adult brain.
Publisher version (URL)https://doi.org/10.1038/s41593-019-0476-2
URIhttp://hdl.handle.net/10261/217851
DOI10.1038/s41593-019-0476-2
ISSN1097-6256
E-ISSN1546-1726
Appears in Collections:(IN) Artículos
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