English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/217800
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Ecdysone-induced 3D chromatin reorganization involves active enhancers bound by Pipsqueak and Polycomb

AuthorsGutierrez-Perez, Irene; Jordan Rowley, M.; Lyu, Xiaowen; Valadez-Graham, Viviana; Vallejo, Diana M.; Ballesta-Illan, Esther; López-Atalaya, José P.; Kremsky, Isaac; Caparrós, Esther; Corces, Victor G.; Domínguez, María
KeywordsChromatin
Transcription
Epigenetics
Hormone
Polycomb
Pipsqueak
Issue Date2019
PublisherElsevier
CitationCell Reports 28(10): 2715-2727.e5 (2019)
AbstractEvidence suggests that Polycomb (Pc) is present at chromatin loop anchors in Drosophila. Pc is recruited to DNA through interactions with the GAGA binding factors GAF and Pipsqueak (Psq). Using HiChIP in Drosophila cells, we find that the psq gene, which has diverse roles in development and tumorigenesis, encodes distinct isoforms with unanticipated roles in genome 3D architecture. The BR-C, ttk, and bab domain (BTB)-containing Psq isoform (PsqL) colocalizes genome-wide with known architectural proteins. Conversely, Psq lacking the BTB domain (PsqS) is consistently found at Pc loop anchors and at active enhancers, including those that respond to the hormone ecdysone. After stimulation by this hormone, chromatin 3D organization is altered to connect promoters and ecdysone-responsive enhancers bound by PsqS. Our findings link Psq variants lacking the BTB domain to Pc-bound active enhancers, thus shedding light into their molecular function in chromatin changes underlying the response to hormone stimulus.
Publisher version (URL)https://doi.org/10.1016/j.celrep.2019.07.096
URIhttp://hdl.handle.net/10261/217800
DOIhttp://dx.doi.org/10.1016/j.celrep.2019.07.096
ISSN2211-1247
Appears in Collections:(IN) Artículos
Files in This Item:
File Description SizeFormat 
pipscomb.pdf4,59 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.